Product Class: Other

Lambda DNA

Product Introduction

Commonly used DNA substrate
Catalog # Size Concentration
N3011L 1,250 μg 500 μg/ml
N3011S 250 μg 500 μg/ml

Product Information

Description

Duplex DNA is isolated from bacteriophage lambda (cI857ind 1 Sam 7). Lambda DNA is 48,502 base pairs in length.

Source

The phage is isolated from the heat-inducible lysogen E. coli l cI857 S7. The DNA is isolated from the purified phage by phenol extraction and dialyzed against 10 mM Tris-HCl (pH 8.0), 1 mM EDTA. 
Product Categories:
DNA Plasmid & Substrates Products

Properties and Usage

Storage Temperature

-20°C

Storage Conditions

10 mM Tris-HCl
1 mM EDTA
pH 8.0 @ 25°C

References

  1. Daniels, D.L. et al. (1983). Appendix II: Complete Annotated Lambda Sequence. R.W. Hendrix, J.W. Roberts, F.W. Stahl and R. A. Weisberg(Ed.), Lambda-II. 519-676. New York: Cold Spring Harbor Laboratory Press.

Faqs & Tech Tips

FAQs

  1. Is the Lambda DNA (N3011) methylated?
  2. What are the Lambda DNA cohesive ends?
  3. Is your Lambda DNA linear or circular?
  4. What are the mutations in your lambda DNA?

Protocols & Manuals

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.)

Other Tools & Resources

Interactive Tools

Quality & Safety

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.