Duplex DNA is isolated from bacteriophage lambda (cI857ind 1 Sam 7). Lambda DNA is 48,502 base pairs in length.
The phage is isolated from the heat-inducible lysogen E.
coli l cI857 S7. The DNA is isolated from the purified phage by
phenol extraction and dialyzed against 10 mM Tris-HCl (pH 8.0), 1 mM EDTA.
Properties and Usage
10 mM Tris-HCl 1 mM EDTA pH 8.0 @ 25°C
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Daniels, D.L. et al. (1983). Appendix II: Complete Annotated Lambda Sequence. R.W. Hendrix, J.W. Roberts, F.W. Stahl and R. A. Weisberg(Ed.), Lambda-II. 519-676. New York: Cold Spring Harbor Laboratory Press.