Duplex DNA is isolated from bacteriophage lambda (cI857ind 1 Sam 7). Lambda DNA is 48,502 base pairs in length.
The phage is isolated from the heat-inducible lysogen E.
coli l cI857 S7. The DNA is isolated from the purified phage by
phenol extraction and dialyzed against 10 mM Tris-HCl (pH 8.0), 1 mM EDTA.
Properties and Usage
10 mM Tris-HCl 1 mM EDTA pH 8.0 @ 25°C
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at firstname.lastname@example.org or fill out the Technical Support Form for appropriate document.
Daniels, D.L. et al. (1983). Appendix II: Complete Annotated Lambda Sequence. R.W. Hendrix, J.W. Roberts, F.W. Stahl and R. A. Weisberg(Ed.), Lambda-II. 519-676. New York: Cold Spring Harbor Laboratory Press.