Product Class: Other

TaqI Methyltransferase
neb31 cloned at NEB recombinant 65 No SAM

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

M0219TaqI_thumb

Product Introduction

  • TaqI Methyltransferase modifies the adenine residue (N6) of the sequence TCGA
  • Supplied with 10X rCutSmart™ Buffer and 32 mM S-adenosylmethionine (SAM)
Catalog # Size Concentration
M0219S 1000.0 units 10000 units/ml

Protocols, Manuals & Usage

Protocols

  1. Recommended Protocol for 3H labeling of DNA
  2. Recommended Protocol for Methylation of Genomic DNA

FAQs & Troubleshooting

FAQs

  1. Is S-adenosylmethionine (SAM) supplied with the Methyltransferase?
  2. What should be considered if the methylation is not going to completion?
  3. What is the activity of TaqI Methyltransferase at 37°C?
  4. What is the activity of TaqI Methyltransferase at 50°C?
  5. Does TaqI Methyltransferase require MgCl2?
  6. Does TaqI Methyltransferase require BSA?
  7. What is the activity of TaqI Methyltransferase in other buffers?
  8. What is the molecular weight of TaqI Methyltransferase?
  9. Is TaqI Methyltransferase used in any special techniques?
  10. Will any NEB methyltransferases (methylases) work on RNA?
  11. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?

Other Products You May Be Interested In