Product Class: Other

SP6 RNA Polymerase
NEBU recombinant 37

Product Introduction

SP6 RNA Polymerase is used for in vitro mRNA synthesis and is highly specific for the SP6 promoter.

Catalog # Size Concentration
M0207S 2000.0 units 20000 units/ml
M0207L 10000.0 units 20000 units/ml

Protocols, Manuals & Usage

Protocols

  1. SP6 In Vitro Transcription Reaction Protocol (M0207)

Application Notes

Tools & Resources

Selection Charts

FAQs & Troubleshooting

FAQs

  1. Does the transcription reaction with SP6 RNA Polymerase require a primer?
  2. Does SP6 RNA Polymerase leave an extra base at the end of a transcript?
  3. Will SP6 RNA Polymerase work on single stranded substrate?
  4. Will SP6 RNA Polymerase work on uncut plasmid DNA?
  5. Can aberrant RNA be produced when using SP6 RNA Polymerase?
  6. Can I use SP6 RNA Polymerase to make high specific activity radiolabeled probes?
  7. Why is the specific activity of the probe low?
  8. What are the main causes of reaction failure using SP6 RNA Polymerase?

Tech Tips

Supplement reactions with fresh DTT if buffer is > 6 months old. 

Increase yields by raising rNTP concentrations to 4 mM each and MgCl2 to 20 mM.

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