Transformation efficiency: 1–5 x 107 cfu/μg pUC19 DNA
Routine non-T7 expression
Deficient in proteases Lon and OmpT
Resistant to phage T1 (fhuA2)
Free of animal products
Chemically competent E. coli cells suitable for transformation and protein expression. This strain does not express the T7 RNA polymerase.
Routine non-T7 expression
fhuA2 [lon] ompT gal [dcm] ΔhsdS
The following reagents are supplied with this product:
Store at (°C)
pUC19 Transformation Control Plasmid
SOC Outgrowth Medium
Properties and Usage
Antibiotics for Plasmid Selection
Ships on dry ice
Quality Control Assays
The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page.
Transformation Efficiency: The competent cells are tested for transformation efficiency and pass minimum release criteria. Transformation efficiency is defined as the number of colony forming units (cfu) which would be produced by transforming 1 μg of plasmid into a given volume of competent cells.
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please contact NEB’s Global Business Development team at firstname.lastname@example.org.
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at email@example.com or fill out the Technical Support Form for appropriate document.
CAUTION: This product contains DMSO, a hazardous material. Review the MSDS before handling.
STORAGE AND HANDLING: Competent cells should be stored at -80°C. Storage at -20°C will result in a significant decrease in transformation efficiency. Cells lose efficiency whenever they are warmed above -80°C, even if they do not thaw.
COMPATIBLE EXPRESSION VECTORS/PROMOTERS: Ptac, Ptrc, Plac, PlacUV5, ParaBAD, PrhaBAD, T5-lacO
Note: Expression from vectors containing a T5-lacO promoter will not be regulated unless the expression vector encodes the lacI gene or a compatible vector expresses LacI or the host strain overproduces LacI (e.g NEB #C3037 NEB Express Iq Competent E. coli)."