B0253 FAQs for M-MuLV Reverse Transcriptase Reaction Buffer, RNA Reagents, Intl, NEB
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M-MuLV Reverse Transcriptase Reaction Buffer FAQ

Q1: What is supplied with the M-MuLV Reverse Transcriptase Reaction Buffer?
Q2: What is the composition of M-MuLV Reverse Transcriptase Reaction Buffer?
Q3: How should the NEBuffer be used?
Q4: The buffer arrived thawed; is it and the enzyme still active?

Q1: What is supplied with the M-MuLV Reverse Transcriptase Reaction Buffer?

A1: The pack contains 4 vials containing a total of 6 ml of 10X M-MuLV Reverse Transcriptase Reaction Buffer.


Q2: What is the composition of M-MuLV Reverse Transcriptase Reaction Buffer?

A2: 1X M-MuLV Reverse Transcriptase Reaction Buffer: 50 mM Tris-HCl, 30 mM KCl, 8 mM MgCl2, 10 mM DTT, pH 8.3 @ 25 C. Supplied as a 10X concentrated stock. Supplement with 0.15 mM each dATP, dTTP, dGTP, dCTP (
NEB# N0446S Nucleotide Solutions - 4 vials 25 μmol each deoxyribonucleotide not supplied).


Q3: How should the NEBuffer be used?

A3: The buffer must be completely thawed before use. Dilute the 10X stock with dH2O to a final concentration of 1X. Add the water first, buffer next, the substrate and finally the enzyme. For example, a 50 l reaction should contain 5 l of 10X buffer with the rest of the volume coming from the substrate, enzyme and dH2O.


Q4: The buffer arrived thawed; is it and the enzyme still active?

A4: The buffers will often thaw when shipped on wet ice. This is often due to the high salt concentration of the 10X buffer. If the ice pack is still solid and the contents are cold to the touch the buffer and enzyme are fine.