Product Pathways - Development
Jagged1 (1C4) Rabbit mAb #2155
|2155S||100 µl (10 western blots)||---||In Stock||---|
|2155||carrier free and custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
Jagged1 (1C4) Rabbit mAb detects endogenous level of total Jagged1 protein. It does not cross-react with Jagged2.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly867 (extracellular region) of human Jagged1.
Notch signaling is activated upon engagement of the Notch receptor with its ligands, the DSL (Delta, Serrate, Lag2) proteins of single-pass type I membrane proteins. The DSL proteins contain multiple EGF-like repeats and a DSL domain that is required for binding to Notch (1,2). Five DSL proteins have been identified in mammals: Jagged1, Jagged2, Delta-like (DLL) 1, 3 and 4 (3). Ligand binding to the Notch receptor results in two sequential proteolytic cleavages of the receptor by the ADAM protease and the γ-secretase complex. The intracellular domain of Notch is released and then translocates to the nucleus where it activates transcription. Notch ligands may also be processed in a way similiar to Notch, suggesting a bi-directional signaling through receptor-ligand interactions (4-6).
Mutation in Jagged1 is associated with Alagille syndrome, an autosomal dominant disorder characterized by abnormal development of liver, heart, skeleton, eye, and face (7, 8) and Tetralogy of Fallot (ToF), a common form of complex congenital heart disease (9). Jagged1 expression is associated with prostate cancer metastasis and recurrence (10).
- Wilson, A. and Radtke, F. (2006) FEBS Lett. 580, 2860-2868.
- Hansson, E.M. et al. (2004) Semin. Cancer Biol. 14, 320-328.
- Chiba, S. (2006) Stem Cells 24, 2437-2447.
- Bland, C.E. et al. (2003) J. Biol. Chem. 278, 13607-13610.
- Six, E. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 7638-7643.
- LaVoie, M.J. and Selkoe, D.J. (2003) J. Biol. Chem. 278, 34427-34437.
- Li, L. et al. (1997) Nat. Genet. 16, 243-251.
- Röpke, A. et al. (2003) Hum. Mutat. 21, 100.
- Eldadah, Z.A. et al. (2001) Hum. Mol. Genet. 10, 163-169.
- Santagata, S. et al. (2004) Cancer Res. 64, 6854-6857.
- Ghosh, S. et al. (2011) J Biol Chem 286, 22678-87. Applications: Western Blotting.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7727 Biotinylated Protein Ladder Detection Pack
- 4147 Cleaved Notch1 (Val1744) (D3B8) Rabbit mAb
- 2421 Cleaved Notch1 (Val1744) Antibody
- 2620 Jagged1 (28H8) Rabbit mAb
- 4608 MAML1 Antibody
- 4618 MAML2 Antibody
- 3447 Notch1 (5B5) Rat mAb
- 3439 Notch1 (C37C7) Rabbit mAb
- 3268 Notch1 (C44H11) Rabbit mAb
- 2423 Notch4 (L5C5) Mouse mAb
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 6883 SignalFire™ ECL Reagent
- 12757 SignalFire™ Elite ECL Reagent
- 12630 SignalFire™ Plus ECL Reagent
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
U.S. Patent No. 5,675,063.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.