Product Pathways - Neuroscience
Plexin A4 (C5D1) Rabbit mAb #3816
|3816S||100 µl (10 western blots)||---||In Stock||---|
|3816||carrier free and custom formulation / quantity||email request|
|W||1:1000||Mouse, Rat||Endogenous||212||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Species predicted to react based on 100% sequence homology: Human.
Specificity / Sensitivity
Plexin A4 (C5D1) Rabbit mAb detects endogenous levels of total Plexin A4 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a GST-fusion protein corresponding to residues from the human sequence of Plexin A4.
Class 3 secreted semaphorin (Sema3A) is a chemorepellent that acts upon a wide variety of axons. As such, it induces a dramatic redistribution and depolymerization of actin filaments that results in growth cone collapse. Plexins are single membrane-spanning signaling proteins encompassing Plexin A1, A2, A3, and A4. Plexins form a complex with neuropilin-1 and -2 and the cell adhesion protein L1 to form a functional semaphorin receptor (1,2). The GTPase Rnd1 binds to the cytoplasmic domain of Plexin A1 to trigger cytoskeletal collapse. In contrast, the GTPase RhoD blocks Rnd1-mediated Plexin A1 activation and repulsion of sympathetic axons by Sema3A (3).
Both Plexin A3 and A4 mediate the responses to class 3 Semaphorins in sensory and sympathetic neurons. In particular, Plexin A4 is responsible for signaling of Sema3A via neuropilin-1, while Plexin A3 is responsible for signaling of Sema3F via neuropilin-2 (4).
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 9998 BSA
- 7727 Biotinylated Protein Ladder Detection Pack
- 9999 Nonfat Dry Milk
- 3813 Plexin A1 Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 9968 Rho-GTPase Antibody Sampler Kit
- 6883 SignalFire™ ECL Reagent
- 12757 SignalFire™ Elite ECL Reagent
- 12630 SignalFire™ Plus ECL Reagent
- 9997 Tris Buffered Saline with Tween® 20 (TBST-10X)
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
U.S. Patent No. 5,675,063.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.