Product Pathways - Neuroscience
Plexin A4 (C5D1) Rabbit mAb #3816
|3816S||100 µl (10 western blots)||---||In Stock||---|
|3816||carrier free and custom formulation / quantity||email request|
|W||1:1000||Mouse, Rat||Endogenous||212||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Species predicted to react based on 100% sequence homology: Human.
Specificity / Sensitivity
Plexin A4 (C5D1) Rabbit mAb detects endogenous levels of total Plexin A4 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a GST-fusion protein corresponding to residues from the human sequence of Plexin A4.
Class 3 secreted semaphorin (Sema3A) is a chemorepellent that acts upon a wide variety of axons. As such, it induces a dramatic redistribution and depolymerization of actin filaments that results in growth cone collapse. Plexins are single membrane-spanning signaling proteins encompassing Plexin A1, A2, A3, and A4. Plexins form a complex with neuropilin-1 and -2 and the cell adhesion protein L1 to form a functional semaphorin receptor (1,2). The GTPase Rnd1 binds to the cytoplasmic domain of Plexin A1 to trigger cytoskeletal collapse. In contrast, the GTPase RhoD blocks Rnd1-mediated Plexin A1 activation and repulsion of sympathetic axons by Sema3A (3).
Both Plexin A3 and A4 mediate the responses to class 3 Semaphorins in sensory and sympathetic neurons. In particular, Plexin A4 is responsible for signaling of Sema3A via neuropilin-1, while Plexin A3 is responsible for signaling of Sema3F via neuropilin-2 (4).
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
U.S. Patent No. 5,675,063.
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