Product Pathways - Development
Phospho-β-Catenin (Ser33/37/Thr41) Antibody #9561
|9561L||300 µl (30 western blots)||---||In Stock||---|
|9561S||100 µl (10 western blots)||---||In Stock||---|
|9561P||40 µl (4 western blots)||---||In Stock||---|
|9561||carrier free and custom formulation / quantity||email request|
|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||92||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Species predicted to react based on 100% sequence homology: Chicken, Xenopus, Zebrafish, Bovine, Dog, Pig.
Specificity / Sensitivity
Phospho-β-Catenin (Ser33/37/Thr41) Antibody detects endogenous levels of β-catenin only when phosphorylated at serines 33, 37 or threonine 41. It does not recognize beta-catenin phosphorylated at other sites.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser33, Ser37 and Thr41 of human β-catenin. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from 293 cells, pretreated with 20 mM LiCl for 30 minutes and then with 50 nM calyculin A, using Phospho-β-Catenin (Ser33/37/Thr41) Antibody (upper) or β-Catenin Antibody #9562 (lower).
β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).
- Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305.
- Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88.
- Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21.
- Amit, S. et al. (2002) Genes Dev 16, 1066-76.
- Liu, C. et al. (2002) Cell 108, 837-47.
- Yanagawa, S. et al. (2002) EMBO J 21, 1733-42.
- Yost, C. et al. (1996) Genes Dev 10, 1443-54.
- Morin, P.J. et al. (1997) Science 275, 1787-90.
- Amit, S. et al. (2002) . Genes and Development 16, 1066-1076. Applications: Western Blotting.
- Hagen, T. et al. (2002) J. Biol. Chem. 277 (26), 23330-23335. Applications: Western Blotting.
- Callow, M.G. et al. (2011) PLoS One 6, e22595. Applications: Western Blotting.
- Bone, H.K. et al. (2011) J Cell Sci 124, 1992-2000. Applications: Western Blotting.
- Trautmann, M. et al. (2013) Oncogene , . Applications: Western Blotting.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 9534 Acetyl-β-Catenin (Lys49) Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7727 Biotinylated Protein Ladder Detection Pack
- 9564 Phospho-β-Catenin (Ser45) Antibody
- 9565 Phospho-β-Catenin (Thr41/Ser45) Antibody
- 6883 SignalFire™ ECL Reagent
- 12757 SignalFire™ Elite ECL Reagent
- 12630 SignalFire™ Plus ECL Reagent
- 9582 β-Catenin (6B3) Rabbit mAb
- 8480 β-Catenin (D10A8) XP® Rabbit mAb
- 9562 β-Catenin Antibody
- 9581 β-Catenin Antibody (Amino-terminal Antigen)
- 9587 β-Catenin Antibody (Carboxy-terminal Antigen)
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.