Product Pathways - MAPK Signaling
Phospho-p38 MAPK (Thr180/Tyr182) Antibody #9211
|9211L||600 µl (60 western blots)||---||In Stock||---|
|9211S||200 µl (20 western blots)||---||In Stock||---|
|9211||carrier free and custom formulation / quantity||email request|
|W||1:1000||Human, Mouse, Rat, Monkey, D. melanogaster, Pig, S. cerevisiae||Endogenous||43||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Species predicted to react based on 100% sequence homology: Hamster, Zebrafish, Bovine.
Specificity / Sensitivity
Phospho-p38 MAPK (Thr180/Tyr182) Antibody detects endogenous levels of p38 MAPK only when activated by phosphorylation at threonine 180 and tyrosine 182. This antibody does not cross-react with the phosphorylated forms of either p42/44 MAPK or SAPK/JNK. It will also react with p38 singly phosphorylated at Thr180 and singly phosphorylated at Tyr182.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Thr180/Tyr182 of human p38 MAPK. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from C6 cells, untreated or anisomycin-treated, and NIH/3T3 cells, untreated or UV-treated, using Phospho-p38 MAPK (Thr180/Tyr182) Antibody (upper) or p38 MAPK Antibody #9212 (lower).
Western blot analysis of extracts from UV-treated NIH/3T3 cells, using Phospho-p38 MAPK (Thr180/Tyr182) Antibody (upper) or control p38 MAPK Antibody #9212 (lower).
Confocal immunofluorescent analysis of HeLa cells -/+ UV light, labeled with Phospho-p38 MAPK (green). Absence of staining in untreated cells (left) and nuclear localization in treated cells (right). Red = Actin filaments (phalloidin).
p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8).
SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).
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- Yang, P. et al. (2010) Nat Immunol 11, 487-94. Applications: Western Blotting.
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- Hsu, J.T. et al. (2007) Am J Physiol Heart Circ Physiol 292, H2982-7. Applications: Western Blotting.
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- Gonda, R.L. et al. (2012) PLoS One 7, e42369. Applications: Western Blotting.
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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