Product Pathways - Tyrosine Kinase / Adaptors
SignalSlide® Phospho-EGF Receptor IHC Controls #8102
|8102S||1 Pack (5 slides)||---||In Stock||---|
|8102||carrier free and custom formulation / quantity||email request|
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Each control slide contains formalin fixed, paraffin-embedded KYSE 450 cells, both untreated and treated with EGF, that serve as a control for Phospho-EGFR immunostaining. Western blot analysis was performed on extracts derived from the same cells to verify the efficacy of the EGF treatment.
To be used with antibodies: 2235, 2237, 3777, 2236, 2234, 4404, 4407, 4267, 9411, 9417, 9416.
Immunohistochemical analysis of paraffin-embedded KYSE450 cells, untreated (left) or treated with EGF (right), using Phospho-EGF Receptor (Tyr1068) (D7A5) XP (TM) Rabbit mAb #3777.
These slides are intended for use in immunohistochemical assays. Please see Related Products for a list of products that can be used with these slides.
- 15019 Animal-Free Blocking Solution (5X)
- 4267 EGF Receptor (D38B1) XP® Rabbit mAb
- 14166 Hematoxylin
- 13079 Immunohistochemistry Application Solutions Kit (Rabbit)
- 5425 Normal Goat Serum
- 2236 Phospho-EGF Receptor (Tyr1068) (1H12) Mouse mAb
- 3777 Phospho-EGF Receptor (Tyr1068) (D7A5) XP® Rabbit mAb
- 2234 Phospho-EGF Receptor (Tyr1068) Antibody
- 1110 Phospho-EGF Receptor (Tyr1068) Blocking Peptide
- 4404 Phospho-EGF Receptor (Tyr1148) Antibody
- 4407 Phospho-EGF Receptor (Tyr1173) (53A5) Rabbit mAb
- 1175 Phospho-EGF Receptor (Tyr1173) Blocking Peptide
- 2235 Phospho-EGF Receptor (Tyr992) Antibody
- 8713 Phospho-PLCγ1 (Ser1248) (D25A9) Rabbit mAb
- 9411 Phospho-Tyrosine Mouse mAb (P-Tyr-100)
- 9417 Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Biotinylated)
- 9416 Phospho-Tyrosine Mouse mAb (P-Tyr-102)
- 8112 SignalStain® Antibody Diluent
- 8125 SignalStain® Boost IHC Detection Reagent (HRP, Mouse)
- 8114 SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
- 8059 SignalStain® DAB Substrate Kit
- 14747 SignalStain® EDTA Unmasking Solution (10X)
- 14177 SignalStain® Mounting Medium
- 9997 Tris Buffered Saline with Tween® 20 (TBST-10X)
The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation (1,2). Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins (3,4). c-Src is involved in phosphorylation of EGFR at Tyr845 (5). The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling (6). Phosphorylation of EGFR at Tyr1045 creates a major docking site for the adaptor protein c-Cbl, leading to receptor ubiquitination and degradation following EGFR activation (7,8). The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 (9). A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provide a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation (2). Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation (10).
- Hackel, P.O. et al. (1999) Curr Opin Cell Biol 11, 184-9.
- Zwick, E. et al. (1999) Trends Pharmacol Sci 20, 408-12.
- Cooper, J.A. and Howell, B. (1993) Cell 73, 1051-4.
- Hubbard, S.R. et al. (1994) Nature 372, 746-54.
- Biscardi, J.S. et al. (1999) J Biol Chem 274, 8335-43.
- Emlet, D.R. et al. (1997) J Biol Chem 272, 4079-86.
- Levkowitz, G. et al. (1999) Mol Cell 4, 1029-40.
- Ettenberg, S.A. et al. (1999) Oncogene 18, 1855-66.
- Rojas, M. et al. (1996) J Biol Chem 271, 27456-61.
- Feinmesser, R.L. et al. (1999) J Biol Chem 274, 16168-73.
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