Product Pathways - Neuroscience
Phospho-CaMKII (Thr286) Antibody #3361
|3361S||100 µl (10 western blots)||---||In Stock||---|
|3361||carrier free and custom formulation / quantity||email request|
|W||1:1000||Human, Mouse, Rat||Endogenous||50||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
Phospho-CaMKII (Thr286) Antibody detects endogenous levels of CaMKII only when phosphorylated at Thr286.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr286 of human CaMKII. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from Neuro2A cells treated with calcium ionophore (A23187, 2 µM) after a 20-minute preincubation with forskolin (30 μM), using Phospho-CaMKII (Thr286) Antibody (upper) or CaMKII Antibody #3362 (lower).
CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T cell receptor signaling (1,2). CaMKII has catalytic and regulatory domains. Ca2+/calmodulin binding to the CaMKII regulatory domain relieves autoinhibition and activates the kinase (3). The activated CaMKII further autophosphorylates at Thr286 to render the kinase constitutively active (3). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286. PKA (protein kinase A) prevents phospho-CaMKII (Thr286) dephosphorylation through an inhibitory effect on PP1 (4).
- Razani, B. et al. (2011) J Biol Chem 286, 30949-61. Applications: Western Blotting.
- Morales-Alamo, D. et al. (2012) J Appl Physiol 113, 917-28. Applications: Western Blotting.
- Morales-Alamo, D. et al. (2013) J Appl Physiol 114, 566-77. Applications: Western Blotting.
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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