Product Pathways - TGF-beta/Smad Signaling
Phospho-Smad2 (Ser465/467) Antibody #3101
|3101L||300 µl (30 western blots)||---||In Stock||---|
|3101S||100 µl (10 western blots)||---||In Stock||---|
|3101||carrier free and custom formulation / quantity||email request|
|W||1:1000||Human, Mouse, Rat||Endogenous||60||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Species predicted to react based on 100% sequence homology: Chicken, Xenopus, Zebrafish.
Specificity / Sensitivity
Phospho-Smad2 (Ser465/467) Antibody detects endogenous levels of Smad2 only when dually phosphorylated at Ser465 and Ser467, and may detect phosphorylated Smad3 at its equivalent site. This antibody does not cross-react with other Smad-related proteins.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser465/467 of human Smad2. Antibodies are purified by protein A and peptide affinity chromatography.
Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 (1-5). Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses (6-8).
Following stimulation by TGF-beta, Smad2 and Smad3 become phosphorylated at their carboxyl termini by the receptor kinase (serines 465 and 467 on Smad2; serines 423 and 425 on Smad3) by TbetaR-I (9-11). Following phosphorylation, Smad2 and Smad3 form a heteromeric complex with the co-smad family member Smad4. These complexes are translocated to the nucleus where they bind DNA and regulate gene transcription.
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- Massagué, J. (1998) Annu Rev Biochem 67, 753-91.
- Whitman, M. (1998) Genes Dev 12, 2445-62.
- Wu, G. et al. (2000) Science 287, 92-7.
- Attisano, L. and Wrana, J.L. (2002) Science 296, 1646-7.
- Moustakas, A. et al. (2001) J Cell Sci 114, 4359-69.
- Abdollah, S. et al. (1997) J. Biol. Chem. 272, 27678-27685.
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- Liu, X. et al. (1997) Proc. Natl. Acad. Sci. 94, 10669-10674.
- Watabe, T. et al. (2003) J. Cell Biol. 163, 1303-1311. Applications: Western Blotting.
- Stove, C. et al. (2004) Oncogene 23, 5330-5339. Applications: Western Blotting.
- Tomita, M. et al. (2004) Oncogene 23, 8272-8281. Applications: Western Blotting.
- Kondo, M. et al. (2004) Cell Death Differ 11, 1092-101. Applications: Western Blotting.
- Nagata, M. et al. (2006) Genes Cells 11, 1267-80. Applications: Western Blotting.
- Ehata, S. et al. (2007) Cancer Res 67, 9694-703. Applications: Western Blotting.
- Louafi, F. et al. (2010) J Biol Chem 285, 41328-36. Applications: Western Blotting.
- Kroening, S. et al. (2010) Am J Physiol Renal Physiol 298, F796-806. Applications: Western Blotting.
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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