Modern cell research is increasingly relying on single cell analysis.
Whether it is cell analysis in microbiology, immune-oncology or drug discovery processes, gentle single cell dissociation from their matrix is a prerequisite for proper single cell analysis.
OLS, your ideal cell research partner, is proud to present to you TIGR, the semi-automated, enzyme-free, fast and reproducible approach of generating pure and individual single cells from tissue samples.
TIGR is compatible with standard cell culture consumables 50 ml centrifuge tubes and standard cell strainer for mechanically, non-enzymatic and parallel processing of tissue samples in a reduced processing time.
TIGR is composed of a benchtop instrument and the grinding tube, a sterile disposable with the integrated core unit and cell strainer. The two-part core unit, consisting of rotor and stator inserts is equipped with counter rotating grinding teeth.
One of the main advantages of TIGR is its flexibility. The process parameters time, direction of rotation, acceleration, speed and the number of process steps can be adjusted by the researcher. Indeed, this allows creating an-experiment specific protocol for every application.
Another major advantage is saving time. The dissociation by TIGR was completed in less than two minutes. This is substantially less when benchmarked against other enzyme based technologies, methods that take 60 minutes, especially when multiple samples are processed in parallel.
Highlights
- Gentle sample preparation
- Enzyme free and standardized single cell dissociation
- Easy to use software and customizable protocols
- Time saving
- Process Control
Tissue Grinder and Dissociator
The grinding unit interlocks with rows of teeth that can be moved against each other. The free rotation of the grinding teeth draws the tissue sample into the free space applying adequate amounts of shearing and milling force to gently isolate single cell from the tissue. The space between the grinding teeth and their fin-shaped geometry allows efficient extraction of viable single cells.
Validated Tissue Types
| Liver |
Muscle |
| Spleen Lung |
Kidney |
| Skin |
Cartilage |
| Lymph Nodes |
Bone |
| FFPE Samples |
Applications
- 3D Cell Culture
- Tissue Models – Spheroids, Organoids, Tumoroids
- Single Cell Counting
- Isolation of Primary Cells
- Cancer Cell Line Development
- Flow Cytometry
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