CASY - Cell Counter and Analyzer
The international Gold Standard* for label-free, non-invasive determination of cell number and viability. This flexible and reliable cell analyzer joins perfect reproducibility and robustness into one instrument, combined with a life time calibration and nearly zero maintenance. The well proven system for fast, cost effective and yet simple to operate multi-parameter analysis of cell cultures.
CASY® system determines the following parameters within seconds only:
Cell Number – Total cell count and live cell count
Cell Viability – Non-invasive counting, no dyes or stains used
Cell Size Distribution – High resolution and large dynamic measuring range
Cell Volume – Calculation of the average cell diameter
Cell Aggregation – Measure and calculates cell aggregation factor, automatic correction possible
Cell Debris – Quality control of cell culture solutions
CASY® Cell Counter works fully automated. Stains or dyes become obsolte – no interferences from dyes, combined with minimum work effort.
Principle of Function
The CASY® technology uses the current exclusion method: particles and cells passing through a defined size pore generate a resistance signal varying with their size and conductivity. Live cells with a polarized membrane generate a high resistance signal, whereas dying or dead cells display a much lower resistance due to their depolarized or fully disrupted cell membrane. The signal of one cell passing the pore is resolved with a high frequency of 1 MHz, and hence the area under the curve = the Integral can easily be calculated, the integral being the only parameter directly proportional to the cell size. As the integral is seriously different with live cells, dead cells and debris, the particle sizes and their distribution can be recorded and analyzed very precisely. One additional benefit is the large dynamic measuring range (e.g. 150µ capillary: 3.2 – 120 µm = from debris via dead/live cells to cell aggregates of several 100 cells size), which cannot be reached by any other method of cell size analysis. By limiting the number of count events, CASY® works without any mathematical approximations to deal with multiple particles passing the capillary simultaneously, every cell or particle is analyzed as a single count event.
Reliability and Versatility
CASY Cell Counters and Analysers combine all advantages of non-invasive, dye-free cell analysis with an ultra-durable hardware, easy to operate and nearly maintenance free. Inter-system and inter-laboratory reproducibility is guaranteed. By use of three different counting capillaries a size range from as low as 0.8 µm to 120 µm, all cell types from algae, bacteria, yeast to prokaryotic and eukaryotic cells can be analysed with one system only.
*More than 2000 publications refer to the CASY systems, as well as numerous diploma and PhD theses. The CASY systems have taken their place in academic and industrial research and process development. The CAYSY Technology: more than 20 years of unprecedented performance in Cell Counting and Analysis
The supporting documents available for this product can be downloaded below.