Product Class: Other

Monarch® HMW gDNA Tissue Lysis Buffer


Catalog #T3061

Product Introduction

  • Supplied as a component of the Monarch HMW DNA Extraction Kit for Tissue (NEB #T3060)
  • This lysis buffer enables rapid and effective lysis of various tissue and bacteria samples
  • Offers maximal protection against nucleases allowing for the isolation of intact HMW DNA

Product Information

Description

The Monarch HMW gDNA Tissue Lysis Buffer, supplied as a component of the Monarch HMW DNA Extraction Kit for Tissue (NEB #T3060), is a highly effective lysis buffer for tissue and bacteria samples. Its composition ensures immediate and complete inactivation of nucleases in the samples at the start of the lysis.
This product is related to the following categories:
Genomic DNA Extraction & Purification Products,
Nucleic Acid Purification Products,

Properties & Usage

Protocols, Manuals & Usage

Protocols

  1. Download Quick Protocol Card for Tissue
  2. Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Tissue (NEB #T3060)
  3. Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Bacteria (NEB #T3060)
  4. Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Yeast (NEB #T3060)
  5. Protocol for UHMW DNA Cleanup in the Oxford Nanopore Technologies® UL Library Prep Workflow

Application Notes

FAQs & Troubleshooting

FAQs

  1. Can I use the Monarch HMW DNA Extraction Kits for processing plant samples?
  2. Can I use the Monarch HMW DNA Extraction Kits for processing insect samples?
  3. Can the Monarch HMW DNA Extraction Kit be used for processing yeast and/or fungal samples?
  4. Can the Monarch HMW DNA Extraction Kits be used to process marine samples, including marine invertebrates?
  5. Can the Monarch HMW DNA Extraction Kits be used to process environmental or fecal samples for metagenomic studies?
  6. Can the Monarch HMW DNA Extraction Kits be used to isolate BAC DNA?
  7. How do you suggest homogenizing tougher tissues, like ear punches or muscle? Can a sonicator be used for tissue homogenization?
  8. Does the kit work with frozen tissue, tissue stored in preservatives (ethanol, DMSO-salt/DESS, or RNAlater®), FFPE samples, or OCT-preserved tissues?
  9. Do you see different fragmentation results when using the pestle versus the rotor-stator homogenizer for tissue homogenization?

Troubleshooting

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Licenses

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information about commercial rights, please email us at [email protected] While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

Other Products You May Be Interested In