Monarch^® DNA Capture Beads

Catalog #T3005

Product Introduction

Specialized 4 mm borosilicate glass beads
Smooth surface enables easy and efficient elution, and HMW DNA is ready to use soon after extraction
Optimized for the purification of high molecular weight (HMW) DNA in the Monarch HMW DNA extraction workflows
Use with the Monarch Bead Retainers (NEB #T3004) and Monarch Collection Tubes II (NEB #T2018) for best results

Check out our publication in Biotechniques outlining a simple approach to accurate quantitation of ultra high molecular weight (UHMW) DNA.

Product Information

Description

Monarch DNA Capture beads are provided as a component of the Monarch HMW DNA Extraction Kits (NEB #T3050 and #T3060). The specialized beads are 4 mm in diameter and made from borosilicate glass and have been optimized for capturing high molecular weight DNA in the Monarch HMW DNA Extraction workflows. The use of large glass beads in the HMW DNA extraction workflow enables fast and efficient washing with no drying steps required. Elution from the beads is fast and complete; all or nearly all of the DNA is recovered. DNA eluted from the beads is also easy to dissolve and ready to use in significantly less time than other HMW DNA extraction methods. The beads should be used in conjunction with the Monarch Bead Retainers (NEB #T3004) and Monarch Collection Tubes II (NEB #T2018) for convenient handling and for best results.

NEB #T3050: 10 μg lambda DNA (~20 μl, NEB #N3011) was combined with Monarch gDNA Nuclei Prep Buffer to bring the total volume to 50 μl. The Cell Protocol (low input) was carried out from Step 4, omitting enzymes and enzyme incubation steps. NEB #T3060: 10 μg lambda DNA (~20 μl, NEB #N3011) was combined with Monarch HMW gDNA Tissue Lysis Buffer to a total volume of 100 μl. The Tissue Protocol (for ‘very low input’ samples) was carried out from Step 8, while omitting enzymes and enzyme incubation steps. DNA concentration of 100 μl eluates from each kit was measured by OD assessment. Average recovery rate and standard deviation were calculated based on 8-fold replicates.

This product is related to the following categories:: Genomic DNA Extraction & Purification Products,; Nucleic Acid Purification Products,

Properties & Usage

Protocols, Manuals & Usage

Protocols

Usage & Guidelines

Application Notes

Monarch^® HMW DNA Extraction Kit improves sample preparation for Oxford Nanopore Technologies^® sequencing of malaria parasites

FAQs & Troubleshooting

FAQs

Troubleshooting

Troubleshooting Guide for Monarch HMW DNA Extraction Kits (NEB #T3050 and #T3060)

Citations & Technical Literature

Citations

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

T3005L_v2

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Licenses

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information about commercial rights, please email us at [email protected] While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

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