Product Class: Restriction Endonuclease

Nb.BtsI
neb31 cloned at NEB recombinant dil_A 37 80 Heat

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

NEB restriction endonuclease that recognizes the sequence GCAGTG_NN

Product Introduction

  • 100% activity in rCutSmart Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests
  • This is a nicking endonuclease
  • Generates DNA molecules that are “nicked”, rather than cleaved
  • Nicking Enzyme Cut Site: GCAGTG (none/0)
Catalog # Size Concentration
R0707S 1000.0 units 10000 units/ml
R0707L 5000.0 units 10000 units/ml

Protocols, Manuals & Usage

Protocols

  1. Optimizing Restriction Endonuclease Reactions
  2. Restriction Digest Protocol
  3. Double Digest Protocol with Standard Restriction Enzymes

Usage & Guidelines

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. What is the activity of nicking enzymes at different temperatures?
  2. Is this enzyme sensitive to dam, dcm or mammalian CpG methylation?
  3. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?

Troubleshooting

Tech Tips

Also has 100% activity in NEBuffer 2
Add 0.4% SDS before running agarose gel to dissociate DNA-enzyme complex