Product Class: Restriction Endonuclease

PciI
neb31 cloned at NEB recombinant dil_B 37 80 Heat

PciI has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10161946. Learn more.

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.


NEB restriction endonuclease that recognizes the sequence A^CATG_T

Product Introduction

Catalog # Size Concentration
R0655S 200.0 units 10000 units/ml
R0655L 1000.0 units 10000 units/ml

Product Information

Description

Product Source

An E.coli strain that carries the cloned PciI gene from Planococcus citreus SE-F45 (S.K. Degtyarev).
This product is related to the following categories:
Restriction Endonucleases P R Products
This product can be used in the following applications:
Restriction Enzyme Digestion

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to digest 1 µg of pXba DNA in 1 hour at 37°C in a total reaction volume of 50 µl.

Reaction Conditions

1X NEBuffer™ r3.1
Incubate at 37°C

1X NEBuffer™ r3.1
100 mM NaCl
50 mM Tris-HCl
10 mM MgCl2
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)

Activity in NEBuffers

NEBuffer™ r1.1: 50%
NEBuffer™ r2.1: 75%
NEBuffer™ r3.1: 100%
rCutSmart™ Buffer: 50%

Diluent Compatibility

Storage Buffer

10 mM Tris-HCl
300 mM NaCl
1 mM DTT
0.1 mM EDTA
500 µg/ml Recombinant Albumin
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation

80°C for 20 minutes

Methylation Sensitivity

dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Not Sensitive

Isoschizomers

BspLU11I+
PscI

Product Notes

  1. PciI is an isoschizomer of BspLU11I.
  2. May exhibit Star Activity in rCutSmart Buffer.
  3. Not sensitive to CpG, dcm, or dam methylation.

Protocols, Manuals & Usage

Protocols

  1. Optimizing Restriction Endonuclease Reactions
  2. Restriction Digest Protocol
  3. Double Digest Protocol with Standard Restriction Enzymes

Usage & Guidelines

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. How can I search for a restriction enzyme by sequence, overhang or name?
  2. How should I stop my restriction digest?
  3. How stable is a particular restriction enzyme?
  4. When should I choose the HF version of an enzyme?
  5. When is star activity a concern?
  6. How should I set up a restriction digest?
  7. I don't see any cleavage after my restriction digest. What factors can interfere with cleavage?
  8. How can I generate a restriction enzyme site map for my sequence?
  9. What information is available in the Restriction Enzyme Database (REBASE)?
  10. Is extended digestion (incubation times > 1 hour) recommended?
  11. Do degenerate recognition sites need to be palindromic?
  12. What does it mean to be Time-Saver™ qualified?
  13. Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)?
  14. Is this enzyme sensitive to dam, dcm or mammalian CpG methylation?
  15. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?
  16. Can Gel Loading Dye, Purple 6X (B7024) be stored in cold temperatures?

Troubleshooting

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specification Change Notifications

PciI has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10161946. All subsequent (higher number) lots will contain rAlbumin. For additional information, please visit at NEB Restriction Enzyme formulations with Recombinant Albumin (rAlbumin).

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.