Product Class: Restriction Endonuclease

NcoI
neb31 cloned at NEB recombinant time saver dil_A 37 80 Heat

NcoI  has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10161944. Learn more.

NcoI has an HF version supplied with CutSmart Buffer. Now comes supplied with 1 vial of Gel Loading Dye Purple (6X) 

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

NEB restriction endonuclease that recognizes the sequence C^CATG_G

Product Introduction

Catalog # Size Concentration
R0193S 1000.0 units 10000 units/ml
R0193T 1000.0 units 50000 units/ml
R0193L 5000.0 units 10000 units/ml
R0193M 5000.0 units 50000 units/ml

Protocols, Manuals & Usage

Protocols

  1. Optimizing Restriction Endonuclease Reactions
  2. Restriction Digest Protocol
  3. Double Digest Protocol with Standard Restriction Enzymes

Usage & Guidelines

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. What is Star Activity and how can it be avoided?
  2. Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
  3. Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)?
  4. Is this enzyme sensitive to dam, dcm or mammalian CpG methylation?
  5. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?

Troubleshooting