EcoRI
Product information| Code | Name | Size | Quantity | Price | |
|---|---|---|---|---|---|
R0101S |
EcoRI, recombinant |
10.000 units ( 20000 units/ml ) | - | Unavailable in your region | |
R0101T |
EcoRI, recombinant, conc. |
10.000 units ( 100000 units/ml ) | - | Unavailable in your region | |
R0101L |
EcoRI, recombinant |
50.000 units ( 20000 units/ml ) | - | Unavailable in your region | |
R0101M |
EcoRI, recombinant, conc. |
50.000 units ( 100000 units/ml ) | - | Unavailable in your region |
EcoRI
EcoRI has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10227213. Learn more.
We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.
- Time-Saver™ qualified for digestion in 5-15 minutes
- High Fidelity (HF®) version available (NEB #R3101) supplied with rCutSmart™ Buffer
- Now comes supplied with 1 vial of Gel Loading Dye, Purple (6X)
- Restriction Enzyme Cut Site: G/AATTC
Featured Video
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Product Information
EcoRI has a High Fidelity version EcoRI-HF® (NEB #R3101).
High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
For details on NEB’s quality controls for restriction endonucleases, visit our Restriction Enzyme Quality page.Product Source
An E. coli strain that carries the cloned EcoRI gene from E. coli RY13 (R.N. Yoshimori).- This product is related to the following categories:
- Restriction Endonucleases C G,
- Time-Saver Qualified Restriction Enzymes
- This product can be used in the following applications:
- Fast Cloning: Accelerate your cloning workflows with reagents from NEB,
- Restriction Enzyme Digestion
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Reagents Supplied
The following reagents are supplied with this product:
NEB # Component Name Component # Stored at (°C) Amount Concentration -
R0101S -20 EcoRI R0101SVIAL -20 1 x 0.5 ml 20,000 units/ml NEBuffer™ EcoRI/SspI B0101SVIAL -20 1 x 1.25 ml 10 X Gel Loading Dye, Purple (6X) B7024AVIAL 25 1 x 0.5 ml 6 X
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R0101T -20 EcoRI R0101TVIAL -20 1 x 0.1 ml 100,000 units/ml NEBuffer™ EcoRI/SspI B0101SVIAL -20 1 x 1.25 ml 10 X Gel Loading Dye, Purple (6X) B7024AVIAL 25 1 x 0.5 ml 6 X
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R0101L -20 EcoRI R0101LVIAL -20 2 x 1.25 ml 20,000 units/ml NEBuffer™ EcoRI/SspI B0101SVIAL -20 2 x 1.25 ml 10 X Gel Loading Dye, Purple (6X) B7024AVIAL 25 1 x 0.5 ml 6 X
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R0101M -20 EcoRI R0101MVIAL -20 1 x 0.5 ml 100,000 units/ml NEBuffer™ EcoRI/SspI B0101SVIAL -20 1 x 1.25 ml 10 X Gel Loading Dye, Purple (6X) B7024AVIAL 25 1 x 0.5 ml 6 X
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Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl.
Reaction Conditions
1X NEBuffer™ EcoRI/SspI
Incubate at 37°C1X NEBuffer™ EcoRI/SspI
100 mM Tris-HCl
50 mM NaCl
10 mM MgCl2
0.025% Triton® X-100
(pH 7.5 @ 25°C)Activity in NEBuffers
NEBuffer™ r1.1: 25%
NEBuffer™ r2.1: 100%
NEBuffer™ r3.1: 50%
rCutSmart™ Buffer: 50%
Diluent Compatibility
Storage Buffer
10 mM KPO4
300 mM NaCl
0.1 mM EDTA
200 µg/ml Recombinant Albumin
50% Glycerol
1 mM DTT
0.15% Triton® X-100
pH 7 @ 25°CHeat Inactivation
65°C for 20 minutesMethylation Sensitivity
dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Blocked by Some Combinations of Overlapping
Isoschizomers
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Related Products
Companion Products
Materials Sold Separately
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Product Notes
- May exhibit star activity in NEBuffer r2.1 or rCutSmart Buffer.
- This enzyme has shown to have lower activity on some supercoiled plasmids, with more than 1 unit required to digest 1 μg plasmid DNA. For complete digestion of 1 μg of plasmid DNA please follow our recommended digestion protocol..
- Blocked by some combinations of overlapping CpG methylation.
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Protocols, Manuals & Usage
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Protocols
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Usage Guidelines
- Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
- Activity of Restriction Enzymes in PCR Buffers
- Cleavage Close to the End of DNA Fragments
- Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
- Double Digests
- Effects of CpG Methylation on Restriction Enzyme Cleavage
- Heat Inactivation
- NEBuffer Activity/Performance Chart with Restriction Enzymes
- Optimizing Restriction Endonuclease Reactions
- Restriction Endonucleases - Survival in a Reaction
- Restriction Enzyme Diluent Buffer Compatibility
- Restriction Enzyme Tips
- Single Letter Codes
- Site Preferences
- Star Activity
- Traditional Cloning Quick Guide
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Tools & Resources
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Selection Charts
- Alphabetized List of Recognition Sequences
- Cleavage of Supercoiled DNA
- Compatible Cohesive Ends and Generation of New Restriction Sites
- Dam-Dcm and CpG Methylation
- Frequencies of Restriction Sites
- Isoelectric Points (pI) for Restriction Enzymes
- Isoschizomers
- NEB Diluent and Buffer Table
- Recleavable Filled-in 5' Overhangs
- Why Choose Recombinant Enzymes?
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Web Tools
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FAQs & Troubleshooting
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FAQs
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Troubleshooting
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Citations & Technical Literature
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Citations
Product Citation Tool
Additional Citations
- Tong C, Li H, Wang Y, Li X, Ou J, Wang D, Xu H, Ma C, Lang X, Liu G, Zhang B, Shi J. (2016) Construction of High-Density Linkage Maps of Populus deltoides × P. simonii Using Restriction-Site Associated DNA Sequencing. PLoS One; Mar 10;11(3), e0150692.
- Yang H, Wei CL, Liu HW, Wu JL, Li ZG, Zhang L, Jian JB, Li YY, Tai YL, Zhang J, Zhang ZZ, Jiang CJ, Xia T, Wan XC. (2016) Genetic Divergence between Camellia sinensis and Its Wild Relatives Revealed via Genome-Wide SNPs from RAD Sequencing. PLoS One; Mar 10;11(3), e0151424.
- Mousavi M, Tong C, Liu F, Tao S, Wu J, Li H, Shi J. (2016) De novo SNP discovery and genetic linkage mapping in poplar using restriction site associated DNA and whole-genome sequencing technologies. BMC Genomics; Aug 18;17, 656.
- Xiao B, Tan Y, Long N, Chen X, Tong Z, Dong Y, Li Y. (2015) SNP-based genetic linkage map of tobacco (Nicotiana tabacum L.) using next-generation RAD sequencing. J Biol Res (Thessalon); Oct 6;22:11. ,
- Zhang Q, Liu C, Liu Y, VanBuren R, Yao X, Zhong C, Huang H. (2015) High-density interspecific genetic maps of kiwifruit and the identification of sex-specific markers. DNA Res; Oct;22(5), 367-75.
- Bonatelli IA, Carstens BC, Moraes EM. (2015) Using Next Generation RAD Sequencing to Isolate Multispecies Microsatellites for Pilosocereus (Cactaceae). PLoS One; Nov 11;10(11), e0142602.
- Fischer G, Azorsa F, Garcia FH, Mikheyev AS, Economo EP. (2015) Two new phragmotic ant species from Africa: morphology and next-generation sequencing solve a caste association problem in the genus Carebara Westwood. Zookeys; Oct 5;(525), 77-105.
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Quality, Safety & Legal
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Quality Control Assays
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here. -
Specifications & Change Notifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]EcoRI has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10227213. All subsequent (higher number) lots will contain rAlbumin. For additional information, please visit at NEB Restriction Enzyme formulations with Recombinant Albumin (rAlbumin).
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Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]- R0101S_L_v1_0341311
- R0101S_L_v1_0331301
- R0101S_L_v1_0331305
- R0101S_L_v1_0331311
- R0101T_M_v1_0331301
- R0101T_M_v1_0331305
- R0101T_M_v1_0341311
- R0101T_M_v1_0341405
- R0101S_L_v1_0341409
- R0101T_M_v1_0341410
- R0101S_L_v1_0341405
- R0101S_L_v2_0341412
- R0101S_L_v1_0341503
- R0101S_L_v2_0341504
- R0101T_M_v2_0341504
- R0101T_M_v2_0341509
- R0101S_L_v2_0341510
- R0101S_L_v2_0341602
- R0101S_L_v2_0341604
- R0101T_M_v2_0341604
- R0101T_M_v2_0341610
- R0101S_L_v2_0341701
- R0101S_L_v2_0341704
- R0101T_M_v2_0351706
- R0101T_M_v2_0351710
- R0101S_L_v2_0351711
- R0101T_M_v2_0351801
- R0101S_L_v2_0351804
- R0101S_v2_10014948
- R0101M_v2_10008581
- R0101M_v2_10019709
- R0101S_v2_10012435
- R0101L_v2_10026006
- R0101S_v2_10026003
- R0101L_v2_10030777
- R0101M_v2_10031413
- R0101S_v2_10033228
- R0101T_v2_10031415
- R0101S_v2_10033233
- R0101S_v2_10046965
- R0101M_v2_10048681
- R0101L_v2_10052727
- R0101T_v2_10050619
- R0101S_v2_10052840
- R0101S_v2_10057096
- R0101L_v2_10060022
- R0101S_v2_10061061
- R0101T_v2_10061321
- R0101M_v2_10064285
- R0101S_v2_10066822
- R0101L_v2_10078628
- R0101S_v2_10081658
- R0101M_v2_10079035
- R0101T_v2_10087360
- R0101S_v2_10087359
- R0101S_v2_10092924
- R0101L_v2_10095559
- R0101S_v2_10095558
- R0101T_v2_10104704
- R0101M_v2_10109723
- R0101L_v2_10119696
- R0101L_v3_10126695
- R0101S_v3_10129803
- R0101T_v3_10130773
- R0101M_v3_10131858
- R0101M_v3_10135001
- R0101T_v3_10135003
- R0101S_v3_10140539
- R0101L_v3_10140538
- R0101M_v3_10150306
- R0101T_v3_10154599
- R0101L_v3_10156282
- R0101S_v3_10160128
- R0101L_v3_10173402
- R0101S_v3_10173400
- R0101T_v3_10173186
- R0101S_v3_10181362
- R0101S_v3_10186608
- R0101M_v3_10191644
- R0101S_v3_10195706
- R0101L_v3_10206909
- R0101S_v3_10206908
- R0101T_v3_10191647
- R0101T_v3_10224155
- R0101S_v3_10215912
- R0101M_v3_10225991
- R0101L_v4_10227225
- R0101M_v4_10227222
- R0101S_v4_10227230
- R0101T_v4_10227231
- R0101S_v4_10242547
- R0101M_v4_10262102
- R0101T_v4_10265611
- R0101L_v4_10270206
- R0101S_v4_10270205
- R0101S_v4_10272276
- R0101S_v4_10288335
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Safety Data Sheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely. -
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email [email protected].
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
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Featured Videos
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Reduce Star Activity with High-Fidelity Restriction Enzymes
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TIME-SAVER™ Protocol for Restriction Enzyme Digests
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NEB® TV Ep. 15 – Applications of Restriction Enzymes
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Restriction Enzyme Digest Protocol: Cutting Close to DNA End
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Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel
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Why is My Restriction Enzyme Not Cutting DNA?
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Restriction Enzyme Digest Problem: Too Many DNA Bands
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Double Digestion with NEBcloner
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The supporting documents available for this product can be downloaded below.
Protocol
Restriction Digest Protocol
Protocol
Restriction Digest Protocol
Protocol
Restriction Digest Protocol