Product Class: Nucleic Acid Marker

Fast DNA Ladder

Now supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS (B7025).
Catalog #SizeConcentrationGel Lanes
N3238S1 ml25 μg/ml50


The Fast DNA ladder is a pre-mixed, ready-to-load molecular weight marker containing xylene cyanol FF dye as a tracking dye. 

The DNA Ladder consists of proprietary plasmids, which are digested to completion with appropriate restriction enzymes to yield 11 bands suitable for use as molecular weight standards for fast electrophoresis systems as well as standard electrophoresis. The digested DNA includes fragments ranging from 50 base pairs to 10 kilobases. The 1 kb fragment has increased intensity to serve as reference band.


Fast DNA Ladder. Mass values are for 0.5 μg/lane

Properties and Usage



Effective Size Range

50bp to 10,002bp

Storage Temperature


Storage Conditions

0.002% Xylene Cyanol
5 mM Tris-HCl
5% Glycerol
pH 8.0 @ 25°C

Supporting Documents

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at or fill out the Technical Support Form for appropriate document.


  1. Preparation: The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.
  2. Usage Recommendation: For standard electrophoresis applications, we recommend loading 20 µl (0.5 µg) of the Fast DNA Ladder per gel lane. The best separation occurs on a 1.2% agarose gel. Below 1%, the 50 bp fragment may not separate from the 150 bp fragment. For use on a fast electrophoresis system, follow the loading recommendations of the system’s manufacturer (5 to 20 µl). The Fast DNA Ladder is suitable for use on the FlashGel® System from Lonza (1.2% gel) or on the E-Gel® system from Invitrogen (1.2 or 2% gels). The Fast DNA Ladder is not intended for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
  3. Fast DNA Ladder is stable for at least 6 months at 25°C.
  4. For long term storage. Store at 4°C or -20°C. If stored at -20°C, mix well after thawing.


  1. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Cold Spring Harbor(Ed.), Molecular Cloning: A Laboratory Manual, (2nd ed.). 10.51-10.67. Cold Spring Harbor Laboratory Press.
  1. How much Fast DNA Ladder should I load on a gel?
  2. What percentage of agarose gel should I use?
  3. Can I store it at -20°C?
  4. Can I use GelRed with the DNA Ladders from NEB?
  5. Can I use SYBR® with the DNA Ladders from NEB?
  6. Why are there extra bands visible on polyacrylamide gels?
  7. Which NEB DNA ladder do you recommend for use with the E-Gel® Precast Agarose Electrophoresis System or The FlashGel™ System?