pUC19 VectorProduct information
DescriptionpUC19 is a commonly used plasmid cloning vector in E.coli. The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number. pUC19 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases (1).
Product SourcepUC19 is isolated from E. coli ER2272 (dam+ dcm+ EcoK M-) by a standard plasmid purification procedure.
Polylinker DNA Sequence
GENBANK Assession NumberL09137
Properties and Usage
Copy NumberHigh (100+)
Origin of Replication
- pMB1 (high-copy mutant)
10 mM Tris-HCl
1 mM EDTA
pH 8.0 @ 25°C
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
- Yanisch-Perron, C., Vieira, J. and Messing, J. (1985). Gene. 33, 103-119.
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