E. coli RNA Polymerase, Holoenzyme
Product information| Code | Name | Size | Quantity | Price | |
|---|---|---|---|---|---|
M0551S |
E. coli RNA Polymerase, Holoenzyme |
50 units ( 1000 units/ml ) | - | Unavailable in your region |
E. coli RNA Polymerase, Holoenzyme
E. coli RNA Polymerase, Holoenzyme is the core enzyme saturated with sigma factor 70. The Holoenzyme initiates RNA synthesis from sigma 70 specific bacterial and phage promoters.
- RNA synthesis from E. coli promoter
- Transcription initiation studies
- In vitro translation with PURExpress®
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Product Information
E. coli RNA Polymerase, Holoenzyme is the core enzyme saturated with sigma factor 70. The Holoenzyme initiates RNA synthesis from sigma 70 specific bacterial and phage promoters.
E. coli RNA Polymerase, Core Enzyme consists of 5 subunits designated α, α, β´, β, and ω. The enzyme is free of sigma factor and does not recognize any specific bacterial or phage DNA promoters. The enzyme retains the ability to transcribe RNA from nonspecific initiation sequences. Addition of sigma factors will allow the enzyme to initiate RNA synthesis from specific bacterial and phage promoters. The core enzyme has a molecular weight of approximately 400 kDa.Product Source
E. coli RNA Polymerase, Holoenzyme is isolated from E. coli strain BL21. The sigma factor 70 is purified from an E. coli strain carrying the cloned gene for sigma factor 70.- This product is related to the following categories:
- RNA Modification,
- RNA Synthesis In vitro Transcription (IVT)
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Reagents Supplied
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Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to incorporate 1 nmol NTP into RNA in 10 minutes at 37°C.Reaction Conditions
1X E.coli RNA Polymerase Reaction Buffer
Incubate at 37°C1X E.coli RNA Polymerase Reaction Buffer
40 mM Tris-HCl
150 mM KCl
10 mM MgCl2
1 mM DTT
0.01% Triton® X-100
(pH 7.5 @ 25°C)Storage Buffer
20 mM Tris-HCl
100 mM NaCl
0.1 mM EDTA
1 mM DTT
50% Glycerol
pH 7.5 @ 25°CUnit Assay Conditions
1X E. coli RNA Polymerase Reaction Buffer, supplemented with 0.5 mM of each NTP and 1 μg T7 phage DNA in 50 μl. -
Advantages and Features
Application Features
- RNA synthesis from E. coli promoter
- Transcription initiation studies
- In vitro translation with PURExpress
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FAQs & Troubleshooting
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Citations & Technical Literature
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Citations
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Quality, Safety & Legal
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Quality Control Assays
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here. -
Specifications & Change Notifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version] -
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number] -
Safety Data Sheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely. -
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email [email protected].
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.Trademarks
New England Biolabs® and PUREXPRESS® are registered trademarks of New England Biolabs, Inc.
TRITON X-100™ is a trademark of The Dow Chemical Company.
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