NEBNext® Quick Ligation ModuleProduct information
Applicable for COVID-19 research
Many scientists are working very hard to gain more knowledge about the SARS-CoV-2 virus (previously 2019-nCoV) and develop diagnostic tests, treatments and vaccines. Crucial questions about this coronavirus need to be answered so quick and reliable research tools matter now more than ever. We at BIOKÉ support the fight against the COVID-19 pandemic by offering a range of high quality products for virus research, from viral purification kits to qPCR plates, together with our reliable technical support. Are you in SARS-CoV-2 research? Let us know how we can support your research and receive a discounted quotation.
Product IntroductionThis module is part of the original standard library prep workflow, which is longer than the NEBNext® Ultra II DNA Library Prep Kit (NEB #E7645) workflow, has more components, and requires more clean-up steps. It also requires higher input amounts: 1 - 5 µg of input DNA vs. 500 pg - 1 µg required for the Ultra II DNA workflow.
The NEBNext® Quick Ligation Module has been optimized to ligate efficiently DNA adaptors compatible with Illumina® sequencing to end-repaired, dA-tailed DNA fragments. The module is optimized for use with the NEBNext End Repair Module (NEB #E6050) and the NEBNext dA-Tailing Module (NEB #E6053) and is part of the original standard DNA library prep workflow, which is suitable for 1 - 5 μg of input DNA.
Each set of reagents is functionally validated together through construction and sequencing of a transcriptome library and sequenced on a
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|Quick T4 DNA Ligase||-20|
|NEBNext Quick Ligation Reaction Buffer||-20|
Properties & Usage
Materials Required but not Supplied
SPRISelect® Reagent Kit (Beckman Coulter® Inc. #B23317) or AMPure® XP Beads (Beckman Coulter, Inc. #A63881)
10 μM Tris HCl pH 7.5-8.0 or 0.1 μM Tris HCl pH 8.0
Protocols, Manuals & Usage
Usage & Guidelines
Tools & Resources
FAQs & Troubleshooting
Citations & Technical Literature
- Resende, P. (2020) Long reads nanopore sequencing to recover SARS-CoV-2 whole genome V.3 protocols.io; DOI: dx.doi.org/10.17504/protocols.io.bfy7jpzn
- Quick, J. (2020) nCoV-2019 sequencing protocol v2 protocols.io; DOI: dx.doi.org/10.17504/protocols.io.bdp7i5rn
- Oxford Nanopore Technologies (2020) PCR Tiling of COVID-19 Virus Oxford Nanopore Technologies Community; Protocol version: PTC_9096_V109_REVF_06FEB2020;
Quality, Safety & Legal
Quality Assurance StatementQuality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
SpecificationsThe Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate Of AnalysisThe Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety DataSheetsThe following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
Quick T4 DNA Ligase
NEBNext Quick Ligation Reaction Buffer
Legal and DisclaimersThis product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
The supporting documents available for this product can be downloaded below.