Product Class: Other

NEBNext® Ultra™ Ligation Module

The small size of this product was discontinued on December 15, 2021. If you would like to purchase a small size, an alternative is NEB #E7595S.

Product Introduction

This module is part of the original Ultra™ workflow, and is optimized for use with the NEBNext® Ultra End Repair/dA-Tailing Module (NEB #E7445), for Illumina®-compatible library construction. The original Ultra workflow enables library preparation from 5 ng – 1 µg of input DNA in a streamlined protocol. However, we now recommend use of the more recent NEBNext Ultra II DNA workflow, which has been further optimized to provide increased yields of high quality libraries from a broader range of input amounts (500 pg – 1 µg), and a NEBNext Ultra II Ligation Module (NEB #E7595) is available.
Catalog # Size Concentration
E7445S 24.0 reactions
E7445L 96.0 reactions

Product Information

Description





 
The NEBNext® Ultra™ Ligation Module has been optimized to ligate efficiently DNA adaptors compatible with Illumina® sequencing to end-repaired, dA-tailed DNA fragments. The module is optimized for use with NEBNext Ultra End Repair/dA-Tailing Module (NEB #E7445), and is part of the original Ultra workflow, which achieves library preparation from 5 ng – 1 µg of input DNA in a streamlined protocol. 

The NEBNext Ultra™ Ligation Module is Designed for Use with the Following Products:
NEBNext Ultra™ End Repair/dA-Tailing Module (NEB #E7442)
NEBNext Singleplex or Multiplex Oligos for Illumina® (NEB #E7350, #E7335, #E7500, #E7710, #E7730, #E6609, #E7600)
NEBNext Q5 Hot Start HiFi PCR Master Mix (NEB #M0543)
NEBNext High-Fidelity 2X PCR Master Mix (NEB #M0541)

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • E7445S     -20    
      NEBNext® Ligation Enhancer E7374AVIAL -20 1 x 0.024 ml Not Applicable
      Blunt/TA Ligase Master Mix E7373AVIAL -20 1 x 0.36 ml Not Applicable
  • E7445L     -20    
      NEBNext® Ligation Enhancer E7374AAVIAL -20 1 x 0.096 ml Not Applicable
      Blunt/TA Ligase Master Mix E7373AAVIAL -20 2 x 0.72 ml Not Applicable

Properties & Usage

Materials Required but not Supplied

  • NEBNext Ultra End Repair/dA-Tailing Module (NEB #E7442)
  • NEBNext Singleplex or Multiplex Oligos for Illumina (NEB #E7350, #E7335, #E7500 or #E7600)
  • NEBNext Q5 Hot Start HiFi PCR Master Mix (NEB #M0543)
  • NEBNext High-Fidelity 2X PCR Master Mix (NEB #M0541)
  • Magnetic rack or plate (e.g., NEBNext® Magnetic Separation Rack (NEB #S1515S), Alpaqua ® 96S Super Magnet Plate (#A001322), or equivalent)

Protocols, Manuals & Usage

Protocols

  1. Where can I find protocols for using NEBNext® Ultra DNA Library Prep Kit and Modules?

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

FAQs & Troubleshooting

FAQs

  1. What is the difference between the NEBNext Ultra Ligation Module and the NEBNext Quick Ligation Module?
  2. How do I obtain the NEBNext Adaptor and USER enzyme for use with the NEBNext Ultra Ligation Module?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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