Yeast Carbon Base Medium PowderProduct information
|12 g||-||Unavailable in your region|
DescriptionThis medium pack includes 12 grams of Yeast Carbon Base Medium Powder and 10 ml of Acetamide Solution.
The Yeast Carbon Base Medium Powder contains sufficient reagents needed to make 1 liter of Yeast Carbon Base (YCB) Agar Medium containing 5 mM acetamide.
YCB Agar Medium is used for acetamide selection of K. lactis cells that have been transformed with a pKLAC1-based expression vector (NEB #N3740). The medium contains glucose and all nutrients needed to sustain growth of K. lactis GG799 cells except a simple nitrogen source. Only transformed cells can utilize acetamide provided in the medium as a source of nitrogen after it is broken down to ammonia by acetamidase (the product of the amdS gene present in pKLAC1).
- Yeast Carbon Base Medium (12g)
- Acetamide solution (sterile) (10 ml)
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|Yeast Carbon Base Medium Powder (12 g)||4|
|Acetamide solution (sterile) (10 ml)||4||100X|
Properties and Usage
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.