Product Class: Other

NEBNext® Quick Ligation Reaction Buffer

Product Introduction

  • Provides optimal conditions for adaptor ligation in standard NEBNext® library prep protocols
  • Not compatible with NEBNext Ultra™ protocols
  • Supplied at 5X concentration
Catalog # Size Concentration
B6058S 2 ml 5 X

Product Information


The 5X NEBNext® dA-Tailing Reaction Buffer is optimized for use with Quick T4 DNA Ligase, in standard NEBNext protocols. This buffer is not compatible with NEBNext Ultra™ protocols.
Product Categories:
Buffer Products
DNA Library Preparation

Properties and Usage

Storage Temperature


1X Master Mix Composition

66 mM Tris-HClpH 7.6 @ -20°C10 mM MgCl2pH 7.6 @ -20°C1 mM DTTpH 7.6 @ -20°C1 mM ATPpH 7.6 @ -20°C6% Polyethylene glycol (PEG 6000)pH 7.6 @ -20°C

1X Master Mix Composition

66mM Tris-HCl (pH 0 @ 0°C)pH 0 @0°C10mM MgCl2 (pH 0 @ 0°C)pH 0 @0°C1mM DTT (pH 0 @ 0°C)pH 0 @0°C1mM ATP (pH 0 @ 0°C)pH 0 @0°C6% Polyethylene glycol (PEG 6000) (pH 0 @ 0°C)pH 0 @0°C

Faqs & Tech Tips

Protocols & Manuals


The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at or fill out the Technical Support Form for appropriate document.)

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Quality & Safety

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.