DNA from FFPE samples
Product informationNucleoSpin® DNA FFPE
DNA recovery from formalin-fixed, paraffin-embedded samples
- Odorless paraffin removal by patented Paraffin Dissolver without xylene
- Efficient removal of crosslinks promotes compatibility with downstream enzymatic reactions
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NucleoSpin® DNA FFPE XS |
NucleoSpin® 8 DNA FFPE |
NucleoSpin® 96 DNA FFPE | |
| Technology | Silica membrane technology | Silica membrane technology | Silica membrane technology |
| Sample Material | ≤ 7 sections (10 μm) of 250 mm² total area (< 15 mg paraffin*) | Tissue (< 10 mg), paraffin (< 15 mg) | Tissue (< 10 mg), paraffin (< 15 mg) |
| Fragment Size | Up to 5 kbp | Up to 5 kbp | Up to 5 kbp |
| Typical Yield | Depending on sample amount and quality | Depending on sample amount and quality | Depending on sample amount and quality |
| Elution Volume | 5–30 μL | 100 μL | 100 μL |
| Binding Capacity | 50 μg | 20 μg | 20 μg |
| Preparation Time | 70 min/6 preps (excl. lysis) | 60 min/6 strips (excl. lysis) | 60 min/plate (excl. lysis) |
* When using the standard protocol with Paraffin Dissolver. Larger quantities of paraffin can be processed when using additional Paraffin Dissolver or the protocol with xylene for deparaffinization.
Application Data
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Reliable DNA yield DNA was purified from seven human ileum as well as human stomach FFPE samples and quantified with Quantifiler® Human DNA Quantification Kit. A DNA yield of approximately 0.3 μg from ileum and 1.7 μg from stomach could be obtained with NucleoSpin® 96 DNA FFPE kit. |
Consistent PCR performance Genomic DNA was isolated with the NucleoSpin® 96 DNA FFPE kit from mouse liver, lung, muscle, and kidney samples (eight FFPE samples each). The purified DNA was quantified with MaximaTM SYBR® Green qPCR Master Mix (amplicon size 100 bp) resulting in reliable CT values indicating consistent DNA yields. |
Ordering information
| Product | Preps | Product code |
| NucleoSpin® DNA FFPE XS | 10 / 50 / 250 | 740980.10 / .50 / .250 |
| NucleoSpin® 8 DNA FFPE | 12 x 8 | 740242 |
| NucleoSpin® 96 DNA FFPE | 1 x 96 / 4 x 96 | 740240.1 / .4 |
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