The NucleoSpin® RNA Clean-up Maxi kit is designed to clean up large amounts of RNA from either crude preparations or from enzyme reactions.
One of the most important aspects during the isolation of RNA is to prevent degradation of the RNA. With the NucleoSpin® RNA Clean-up Maxi kit, RNA containing samples are mixed with a solution containing large amounts of chaotropic ions and ethanol. In this mixture, RNases are immediately inactivated and creates appropriate binding conditions to allow adsorption of RNA to the silica membrane. Three washing steps with two different wash buffers remove impurities. Pure RNA is finally eluted at low ionic strenght conditions with RNase-free water (supplied).The RNA clean up using NucleoSpin® RNA Clean-up Maxi kits can be performed at room temperature. The eluate should be treated with care because RNA is very sensitive to trace contaminations of RNases, often found on general lab ware, fingerprints and dust. Keep RNA frozen at -20 °C for short-term or -70 °C for longterm storage to ensure RNA stability.
NucleoSpin® RNA Clean-up Maxi is the perfect choice for clean-up of crude RNA sample up to 35 mg with excellent recovery
Three different crude RNA eluates have been used as input for further RNA clean-up using NucleoSpin® RNA Clean-up Maxi. The input amount of these crude RNA extracts varies from 35.4 mg to 6.8 mg. After RNA clean up according to the standard protocol, 30.1 mg to 6.6 mg of clean RNA was recovered. That RNA amount is equivalent to a recovery rate from 85 % to 96 %.
The supporting documents available for this product can be downloaded below.