NucleoSpin Dx VirusProduct information
- CE-marked in accordance with EU Directive 98/79/EC
- Fits into in-vitro diagnostic workflows
- Compatible with fresh or frozen serum and plasma treated with EDTA or citrate
- Highly consistent viral RNA and DNA isolation
- Suitable for animal samples
Applicable for COVID-19 research
Many scientists are working very hard to gain more knowledge about the SARS-CoV-2 virus (previously 2019-nCoV) and develop diagnostic tests, treatments and vaccines. Crucial questions about this coronavirus need to be answered so quick and reliable research tools matter now more than ever. We at BIOKÉ support the fight against the COVID-19 pandemic by offering a range of high quality products for virus research, from viral purification kits to qPCR plates, together with our reliable technical support.
Isolation of viral RNA and viral DNA from human plasma or serum samples – for in-vitro diagnostic purposes
The NucleoSpin® Dx Virus kit is a generic system for the isolation and purification of viral nucleic acids from human serum or plasma samples for subsequent in-vitro diagnostic purposes. The kit can be used with fresh and frozen human serum and plasma, stabilized with either EDTA or citrate from common blood collection systems. The kit is designed to be used with any downstream application employing enzymatic amplification and detection of RNA and DNA (e.g., RT-PCR, PCR). The viral nucleic acids isolated and purified with NucleoSpin® Dx Virus can be used in qualitative applications (e.g., RT-PCR or PCR for blood screening) as well as in quantitative applications (e.g., detection of viral load by qPCR) employing diagnostic nucleic acid amplification techniques.
NucleoSpin Dx Virus Procedure
NucleoSpin® Dx Virus is based on proven NucleoSpin® silica-membrane technology and provides an easy way to isolate viral RNA and viral DNA from 150 μl of serum or plasma samples. Purified RNA and DNA are ready to use for downstream amplifications like RT-PCR or PCR. The NucleoSpin® Dx Virus procedure is based on a series of simple steps: First, the serum or plasma samples are lysed in the presence of chaotropic salts. For the purification of viral DNA, Proteinase K is added to the lysis reaction. Lysis buffer and ethanol create appropriate conditions for binding of nucleic acids to the silica membrane of the NucleoSpin® Dx Virus Columns while Carrier RNA improves binding and recovery of low-concentrated viral RNA and DNA. Contaminations (potential PCR inhibitors) like salts, metabolites, and soluble macromolecular cellular components are removed in three washing steps. The nucleic acids are finally eluted in 50 μl low salt buffer or DNase-free water.
|Format||Mini spin columns|
|Sample material||Plasma or serum, fresh or frozen, EDTA or citrate treated|
|Sample volume||150 μl|
|Elution Volume||50 μl|
|Preparation Time||~30 min / 1-6 preps|