The universal reagent for RNA isolation
- No chloroform, no phase separation, easy procedure
- High RNA yield and purity from any sample material
- Small and large RNA in one or in separated fractions
< 1 x 107 cultured cells, bacteria, and yeast,
|Fragment size||Small RNA (10–200 nt). Large RNA (> 200 nt)|
|Typical yield (total RNA)
Typical yield (large RNA)
Liver: 6-8 µg/mg tissue
|Typical RIN (RNA integrity number)||> 9|
|Preparation time||< 1 h|
Low DNA contamination and no phenol carry-over with NucleoZOL
Total RNA was isolated with NucleoZOL and competitor product from 50 mg rat lung tissue.
A DNA contamination was quantified by qPCR (competitor Zol=100 %). Compared to a standard two-phase extraction, only a minimum of DNA is carried over during purification with NucleoZOL.
B Absorption ratio A260/A230 of NucleoZOL shows excellent purity. Low A260/A230 ratios in samples purified with competitor Zol indicate a phenol carry-over.
The supporting documents available for this product can be downloaded below.