Protocols

91882:

Western Blotting,  Immunohistochemistry (Leica® Bond™),  Immunohistochemistry (Paraffin),  Immunofluorescence*

Background

CD80 (B7-1, BB1) and CD86 (B7-2, B70) are members of the B7 family of cell surface ligands that regulate T cell activation and immune responses. CD80 is expressed on activated antigen presenting cells, including dendritic cells, B cells, monocytes, and macrophages. CD86 is expressed on resting monocytes, dendritic cells, activated B lymphocytes, and can be further upregulated in the presence of inflammation (1-3). CD80 and CD86 are ligands for CD28, which functions as a T cell costimulatory receptor. Interaction of CD28 with CD80 or CD86 provides the second signal required for naïve T cell activation, T cell proliferation, and acquisition of effector functions (3-7). Alternatively, CD80 and CD86 also act as ligands to CTLA-4, which results in the downregulation of T cell activity (3,7-9).

1. Freeman, G.J. et al. (1989) J Immunol 143, 2714-22.
2. Azuma, M. et al. (1993) Nature 366, 76-9.
3. Adams, A.B. et al. (2016) J Immunol 197, 2045-50.
4. Linsley, P.S. et al. (1990) Proc Natl Acad Sci U S A 87, 5031-5.
5. Gimmi, C.D. et al. (1991) Proc Natl Acad Sci U S A 88, 6575-9.
6. Harding, F.A. et al. (1992) Nature 356, 607-9.
7. Collins, A.V. et al. (2002) Immunity 17, 201-10.
8. Linsley, P.S. et al. (1991) J Exp Med 174, 561-9.
9. Krummel, M.F. and Allison, J.P. (1995) J Exp Med 182, 459-65.

Application References

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Companion Products

• 7003  20X LumiGLO^® Reagent and 20X Peroxide
• 15019  Animal-Free Blocking Solution (5X)
• 7074  Anti-rabbit IgG, HRP-linked Antibody
• 7727  Biotinylated Protein Ladder Detection Pack
• 14166  Hematoxylin
• 13079  Immunohistochemistry Application Solutions Kit (Rabbit)
• 5425  Normal Goat Serum
• 7071  Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 8112  SignalStain^® Antibody Diluent
• 8114  SignalStain^® Boost IHC Detection Reagent (HRP, Rabbit)
• 14746  SignalStain^® Citrate Unmasking Solution (10X)
• 8059  SignalStain^® DAB Substrate Kit
• 14177  SignalStain^® Mounting Medium
• 9997  Tris Buffered Saline with Tween^® 20 (TBST-10X)

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