CD86 (E2G8P) Rabbit mAb
Product informationCode | Name | Size | Quantity | Price | ||||
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91882S |
CD86 (E2G8P) Rabbit mAb |
100 µl |
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€ 322.00 |
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Product Pathways - Lymphocyte Signaling
CD86 (E2G8P) Rabbit mAb #91882
PhosphoSitePlus® protein, site, and accession data:
CD86
No. | Size | Price | ||
---|---|---|---|---|
91882S | 100 µl | --- | In Stock | --- |
91882 | carrier free and custom formulation / quantity | email request |
Application | Dilution | Species-Reactivity | Sensitivity | MW (kDa) | Isotype |
---|---|---|---|---|---|
W | Human | Endogenous | 60-85 | Rabbit IgG | |
IHC-Bond | |||||
IHC-P | |||||
IF-IC |
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IHC-Bond=IHC-Leica® Bond™, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry)
Protocols
Specificity / Sensitivity
CD86 (E2G8P) Rabbit mAb recognizes endogenous levels of total CD86 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro239 of human CD86 protein.
Western Blotting

Western blot analysis of extracts from various cell lines using CD86 (E2G8P) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
IHC-Leica® Bond™

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using CD86 (E2G8P) Rabbit mAb performed on the Leica® BOND™ Rx.
IHC-Leica® Bond™

Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma using CD86 (E2G8P) Rabbit mAb performed on the Leica® BOND™ Rx.
IHC-Leica® Bond™

Immunohistochemical analysis of paraffin-embedded human tonsil using CD86 (E2G8P) Rabbit mAb performed on the Leica® BOND™ Rx.
IHC-Leica® Bond™

Immunohistochemical analysis of paraffin-embedded human ovarian serous carcinoma using CD86 (E2G8P) Rabbit mAb performed on the Leica® BOND™ Rx.
IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using CD86 (E2G8P) Rabbit mAb.
IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded HDLM-2 cell pellet (left, positive) or HT-29 cell pellet (right, negative) using CD86 (E2G8P) Rabbit mAb.
IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using CD86 (E2G8P) Rabbit mAb (left) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
IF-IC

Confocal immunofluorescent analysis of HDLM-2 cells (left, positive) and HT-29 cells (right, negative) using CD86 (E2G8P) Rabbit mAb (green). Blue = Hoechst 33342 #4082 (fluorescent DNA dye).
Background
CD80 (B7-1, BB1) and CD86 (B7-2, B70) are members of the B7 family of cell surface ligands that regulate T cell activation and immune responses. CD80 is expressed on activated antigen presenting cells, including dendritic cells, B cells, monocytes, and macrophages. CD86 is expressed on resting monocytes, dendritic cells, activated B lymphocytes, and can be further upregulated in the presence of inflammation (1-3). CD80 and CD86 are ligands for CD28, which functions as a T cell costimulatory receptor. Interaction of CD28 with CD80 or CD86 provides the second signal required for naïve T cell activation, T cell proliferation, and acquisition of effector functions (3-7). Alternatively, CD80 and CD86 also act as ligands to CTLA-4, which results in the downregulation of T cell activity (3,7-9).
- Freeman, G.J. et al. (1989) J Immunol 143, 2714-22.
- Azuma, M. et al. (1993) Nature 366, 76-9.
- Adams, A.B. et al. (2016) J Immunol 197, 2045-50.
- Linsley, P.S. et al. (1990) Proc Natl Acad Sci U S A 87, 5031-5.
- Gimmi, C.D. et al. (1991) Proc Natl Acad Sci U S A 88, 6575-9.
- Harding, F.A. et al. (1992) Nature 356, 607-9.
- Collins, A.V. et al. (2002) Immunity 17, 201-10.
- Linsley, P.S. et al. (1991) J Exp Med 174, 561-9.
- Krummel, M.F. and Allison, J.P. (1995) J Exp Med 182, 459-65.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 15019 Animal-Free Blocking Solution (5X)
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7727 Biotinylated Protein Ladder Detection Pack
- 14166 Hematoxylin
- 13079 Immunohistochemistry Application Solutions Kit (Rabbit)
- 5425 Normal Goat Serum
- 7071 Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 8112 SignalStain® Antibody Diluent
- 8114 SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
- 14746 SignalStain® Citrate Unmasking Solution (10X)
- 8059 SignalStain® DAB Substrate Kit
- 14177 SignalStain® Mounting Medium
- 9997 Tris Buffered Saline with Tween® 20 (TBST-10X)
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Tween is a registered trademark of ICI Americas, Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
LEICA is a registered trademark of Leica Microsystems IR GmbH.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
BOND is a trademark of Leica Biosystems Melbourne Pty. Ltd. No affiliation or sponsorship between CST and Leica Microsystems IR GmbH or Leica Biosystems Melbourne Pty. Ltd is implied.
The supporting documents available for this product can be downloaded below.