α-Tubulin (11H10) Rabbit mAb (Alexa Fluor® 555 Conjugate)
Product information| Code | Name | Size | Quantity | Price | |
|---|---|---|---|---|---|
5059S |
α-Tubulin (11H10) Rabbit mAb (Alexa Fluor® 555 Conjugate) |
100 µl (50 tests) | - | Unavailable in your region |
Product Pathways - Cytoskeletal Signaling
α-Tubulin (11H10) Rabbit mAb (Alexa Fluor® 555 Conjugate) #5059
PhosphoSitePlus® protein, site, and accession data:
TUBA1B
| No. | Size | Price | ||
|---|---|---|---|---|
| 5059S | 100 µl (50 tests) | --- | In Stock | --- |
| 5059 | carrier free and custom formulation / quantity | email request | ||
| Application | Dilution | Species-Reactivity | Sensitivity | Isotype |
|---|---|---|---|---|
| IF-IC | 1:50 | Human, Mouse, Rat, Monkey, D. melanogaster, Zebrafish, Bovine, Pig | Endogenous | Rabbit IgG |
Species cross-reactivity is determined by western blot using the unconjugated antibody.
Applications Key: IF-IC=Immunofluorescence (Immunocytochemistry)
Homology
Species predicted to react based on 100% sequence homology: Dog.
Protocols
- 5059:
- Immunofluorescence
Specificity / Sensitivity
α-Tubulin (11H10) Rabbit mAb (Alexa Fluor® 555 Conjugate) detects endogenous levels of total α-tubulin protein. This antibody does not cross-react with recombinant β-tubulin.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human α-tubulin. The antibody was conjugated to Alexa Fluor® 555 under optimal conditions with an F/P ratio of 2-6.
IF-IC
Confocal immunofluorescent analysis of HeLa cells using α-Tubulin (11H10) Rabbit mAb (Alexa Fluor® 555 Conjugate) (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Description
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 555 fluorescent dye and tested in-house for immunofluroescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated α-Tubulin (11H10) Rabbit mAb #2125.
Background
The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
U.S. Patent No. 5,675,063.
DRAQ5 is a registered trademark of Biostatus Limited.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
The supporting documents available for this product can be downloaded below.