SOD1 (71G8) Mouse mAb
Product information| Code | Name | Size | Quantity | Price | |
|---|---|---|---|---|---|
4266T |
SOD1 (71G8) Mouse mAb |
20 µl (2 western blots) | - | Unavailable in your region | |
4266S |
SOD1 (71G8) Mouse mAb |
100 µl (10 western blots) | - | Unavailable in your region |
Product Pathways - Neuroscience
SOD1 (71G8) Mouse mAb #4266
PhosphoSitePlus® protein, site, and accession data:
SOD1
| No. | Size | Price | ||
|---|---|---|---|---|
| 4266S | 100 µl (10 western blots) | --- | In Stock | --- |
| 4266T | 20 µl (2 western blots) | --- | In Stock | --- |
| 4266 | carrier free and custom formulation / quantity | email request | ||
| Application | Dilution | Species-Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|---|
| W | 1:1000 | Human | Endogenous | 18 | Mouse IgG1 |
| IP | 1:50 |
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Protocols
Specificity / Sensitivity
SOD1 (71G8) Mouse mAb detects endogenous levels of SOD1 protein. It does not cross-react with other related proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with full-length recombinant human SOD1 protein.
Western Blotting
Western blot analysis of extracts from various cell lines using SOD1 (71G8) Mouse mAb.
Background
SOD1, Cu/Zn superoxide dismutase, is a major antioxidant enzyme that catalyzes the conversion of superoxide anion to hydrogen peroxide and molecular oxygen (1). SOD1 is ubiquitously expressed and is localized in the cytosol, nucleus and mitochondrial intermembrane space. The SOD1 gene locus is on chromosome 21 in a region affected in Down Syndrome (2). In addition, over 100 distinct SOD1 inherited mutations have been identified in the familial form of amyotrophic lateral sclerosis (ALS), a progressive degenerative disease of motor neurons (3-5). Despite the fact that SOD1 helps to eliminate toxic reactive species, its mutations in ALS have been described as gain-of-function (5). The mechanism by which mutant SOD1 induces the neurodegeneration observed in ALS is still unclear. Mutant SOD1 proteins become misfolded and consequently oligomerize into high molecular weight species that aggregate and end up in proteinaceous inclusions (5).
Application References
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Companion Products
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
The supporting documents available for this product can be downloaded below.