Protocols

2085:

Western Blotting,  Immunoprecipitation (Magnetic),  Immunohistochemistry (Paraffin),  Immunofluorescence,  Immunofluorescence,  Flow

Background

The epidermal growth factor (EGF) receptor is a 170 kDa transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Research studies have shown that somatic mutations in the tyrosine kinase domain of EGF receptor (EGFR) are present in a subset of lung adenocarinomas that respond to EGFR inhibitors, such as gefinitib and erlotinib (1-3). Two types of mutations account for approximately 90% of mutated cases: a specific point mutation, L858R, that occurs in exon 21 and short in-frame deletions in exon 19 (4,5). The most frequent exon 19 deletion is E746-A750, accounting for 90% of lesions at this site, although some rare variants occur.

1. Lynch, T.J. et al. (2004) N Engl J Med 350, 2129-39.
2. Pao, W. et al. (2004) Proc Natl Acad Sci USA 101, 13306-11.
3. Haber, D.A. et al. (2005) Cold Spring Harb Symp Quant Biol 70, 419-26.
4. Kosaka, T. et al. (2004) Cancer Res 64, 8919-23.
5. Riely, G.J. et al. (2006) Clin Cancer Res 12, 7232-41.

Application References

• Simonetti, S. et al. (2010) J Transl Med 8, 135. Applications: IF-P (paraffin).
• Brevet, M. et al. (2010) J Mol Diagn 12, 169-76. Applications: IHC-P (paraffin).
• Kawahara, A. et al. (2010) Clin Cancer Res , . Applications: IHC-P (paraffin), Western Blotting.
• Yu, J. et al. (2009) Clin Cancer Res 15, 3023-8. Applications: IF-P (paraffin), IHC-P (paraffin), Western Blotting.
• Rimkunas, V.M. et al. (2012) Clin Cancer Res 18, 4449-57. Applications: IHC-P (paraffin).

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Companion Products

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• 7727  Biotinylated Protein Ladder Detection Pack
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• 4267  EGF Receptor (D38B1) XP^® Rabbit mAb
• 3197  EGF Receptor (L858R Mutant Specific) (43B2) Rabbit mAb
• 2963  EGF Receptor (T43) Antibody
• 3265  EGF Receptor (V279) Antibody
• 2232  EGF Receptor Antibody
• 6883  SignalFire™ ECL Reagent
• 12757  SignalFire™ Elite ECL Reagent
• 12630  SignalFire™ Plus ECL Reagent
• 8112  SignalStain^® Antibody Diluent

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