EGF Receptor (E746-A750del Specific) (D6B6) XP® Rabbit mAb
Product informationCode | Name | Size | Quantity | Price | |
---|---|---|---|---|---|
2085S |
EGF Receptor (E746-A750del Specific) (D6B6) XP® Rabbit mAb |
100 µl (10 western blots) | - | Unavailable in your region |

Product Pathways - Tyrosine Kinase / Adaptors
EGF Receptor (E746-A750del Specific) (D6B6) XP® Rabbit mAb #2085
PhosphoSitePlus® protein, site, and accession data:
EGFR
No. | Size | Price | ||
---|---|---|---|---|
2085S | 100 µl (10 western blots) | --- | In Stock | --- |
2085 | carrier free and custom formulation / quantity | email request |
Application | Dilution | Species-Reactivity | Sensitivity | MW (kDa) | Isotype |
---|---|---|---|---|---|
W | 1:1000 | Human | Endogenous | 175 | Rabbit IgG |
IP | 1:100 | ||||
IHC-P | 1:100 | ||||
IF-P | 1:250 | ||||
IF-IC | 1:250 | ||||
F | 1:1000 |
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-P=Immunofluorescence (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Protocols
Specificity / Sensitivity
EGF Receptor (E746-A750del Specific) (D6B6) XP® Rabbit mAb detects endogenous levels of EGFR E746-A750del mutant protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to E746-A750del mutant sequence of human EGFR.
Western Blotting

Western blot analysis of extracts from various EGFR expressing cell lines, showing the specificity of detection of mutant EGFR (E746-A750del) protein, using EGF Receptor (E746-A750del Specific) (D6B6) XP® Rabbit mAb (upper), and total EGFR control antibody (lower).
IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded H1975 (left, EGFR L858R positive) and H1650 xenografts (right, EGFR deletion positive) using EGF Receptor (E746-A750del Specific) (D6B6) XP® Rabbit mAb (upper) and total EGFR (D38B1) XP® Rabbit mAb #4267 (lower).
IHC-P (paraffin)

Immunohistochemical analysis of paraffin embedded human lung carcinoma of known mutational status using EGF Receptor (E746-A750del Specific) (D6B6) XP® Rabbit mAb (upper) and total EGFR (D38B1) XP® Rabbit mAb #4267 (lower). EGFR L858R positive lung (left), EGFR deletion positive lung sections (right).
IF-P

Confocal immunofluorescent analysis of paraffin-embedded H3255, H1975, HCC827 and H1650 xenografts using EGF Receptor (E746-A750del Specific) (D6B6) XP® Rabbit mAb (upper, green) and EGFR (D38B1) XP® Rabbit mAb #4267 (lower, green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
IF-IC

Confocal immunofluorescent analysis of HCC827, H1650 and Kyse450 cells using EGF Receptor (E746-A750del Specific) (D6B6) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Flow Cytometry

Flow cytometric analysis of Kyse450 cells (wildtype, blue) and HCC827 cells (exon 19 deletion (E746-A750del, green) using EGF Receptor (E746-A750del Specific) (D6B6) XP® Rabbit mAb.
Background
The epidermal growth factor (EGF) receptor is a 170 kDa transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Research studies have shown that somatic mutations in the tyrosine kinase domain of EGF receptor (EGFR) are present in a subset of lung adenocarinomas that respond to EGFR inhibitors, such as gefinitib and erlotinib (1-3). Two types of mutations account for approximately 90% of mutated cases: a specific point mutation, L858R, that occurs in exon 21 and short in-frame deletions in exon 19 (4,5). The most frequent exon 19 deletion is E746-A750, accounting for 90% of lesions at this site, although some rare variants occur.
Application References
- Simonetti, S. et al. (2010) J Transl Med 8, 135. Applications: IF-P (paraffin).
- Brevet, M. et al. (2010) J Mol Diagn 12, 169-76. Applications: IHC-P (paraffin).
- Kawahara, A. et al. (2010) Clin Cancer Res , . Applications: IHC-P (paraffin), Western Blotting.
- Yu, J. et al. (2009) Clin Cancer Res 15, 3023-8. Applications: IF-P (paraffin), IHC-P (paraffin), Western Blotting.
- Rimkunas, V.M. et al. (2012) Clin Cancer Res 18, 4449-57. Applications: IHC-P (paraffin).
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
DRAQ5 is a registered trademark of Biostatus Limited.
XP is a registered trademark of Cell Signaling Technology, Inc.
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