3D CoSeedis™ System
Product informationModular multi-purpose 3D Cell Co-Culture System
3D CoSeedis™ from abc biopply is a novel scaffold-free 3D cell co-culture system. It consists of a unique agarose-based matrix containing an array of conical microwells. The particular and proprietary topography of this arrangement allows the formation of spheroidal and non-spheroidal cell aggregates in a highly-reproducible and consistent manner. The conically shaped microwells within the array allow precise determination of aggregate volume, hence cell growth. Furthermore, the modular composition of the 3D CoSeedis™ system allows co-culturing of feeder cells that are physically separated from the actual test cells (distance co-culture). Consequently, this set-up counts responsible for the standardisation of protocols under defined conditions.
Single Cell Analysis Systems
Offer pre-validated and standardised systems to extract single cells from previously grown micro organoids. Besides all organoid culture relevant components, these systems also provides a proprietary organoid dissociation solution. Contact us for further details.
Customized Systems
Profit from abc biopply’s extended experiences in the standardisation of 3D CoSeedis™ applications. The customized systems will implement your cell line of choice into the 3D CoSeedis™ platform. Please contact us to discuss your requirements.Key Features of 3D CoSeedis™ Matrix
Modular Set-up
- Contact or distance co-cultures with feeder cells
- Combining up to 3 cell types to analyse interactions
- Serum, serum-reduced or serum-free conditions possible
- Application of tissue specific ECM components
Agarose-based Permeable Matrix
- Allows long term 3D culture of cell aggregates
- Sufficient supply with nutrients, oxygen, and catabolites – efficient disposal of toxic waste products
- Efficient use of physically separated feeder cells (distance co-culture)
- Cultivation in standard or defined media
Multi-purpose Applicability
- Applicable to various cell types
- Supports the formation of spheroidal and non-spheroidal aggregates
- Suitable for high-throughput and high-content applications
Process Directed Workflow Protocols
- Validated protocols
- Standardised and optimised components
- Highly reproducible 3D cell constructs
- Suitable for high-throughput and high-content applications
| Feature | Competition | 3D CoSeedis™ |
|---|---|---|
| Distance co-cultures | - | + |
| Aggregation of non-spheroidal cells | - | + |
| Long term culture | - | + |
| Easy handling | - | + |
| Easy harvesting of spheroids | + | + |
| HTS & HCS compatibility | - / + | + |
| Fully validated workflows/systems | - | + |
Applicable Cell Systems
These cells have been tested successfully for aggregate spheroid formation by our academic partners.
Details are described in the publication: A deep conical agarose microwell array for adhesion independent three-dimensional cell culture and dynamic volume measurement Andreas R. Thomsen et al., Lab Chip, DOI 10.1039/C7LC00832E.
Standardized Protocols for 3D Organoid Systems
The modular composition of the 3D CoSeedis ™ system allows distance co-cultures and, consequently, the standardisation of protocols. abc biopply’s product portfolio contains already standardised protocols for a wide variety of co-cultures, especially for the cultivation of different tumours.
Various application notes have been developed that constantly extend the range of assays in which 3D CoSeedis™ has been successfully implemented. Check out the latest notes:
Request more information about the CoSeedis System
The supporting documents available for this product can be downloaded below.