3D CoSeedis™ System
Product informationModular multi-purpose 3D Cell Co-Culture System
3D CoSeedis™ from abc biopply is a novel scaffold-free 3D cell co-culture system. It consists of a unique agarose-based matrix containing an array of conical microwells. The particular and proprietary topography of this arrangement allows the formation of spheroidal and non-spheroidal cell aggregates in a highly-reproducible and consistent manner. The conically shaped microwells within the array allow precise determination of aggregate volume, hence cell growth. Furthermore, the modular composition of the 3D CoSeedis™ system allows co-culturing of feeder cells that are physically separated from the actual test cells (distance co-culture). Consequently, this set-up counts responsible for the standardisation of protocols under defined conditions.
Products offered by BIOKÉ:
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Single-Packaged Chips |
Multi-Packaged Chips |
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Single Cell Analysis Systems
Offer pre-validated and standardised systems to extract single cells from previously grown micro organoids. Besides all organoid culture relevant components, these systems also provides a proprietary organoid dissociation solution. Contact us for further details.
Customized Systems
Profit from abc biopply’s extended experiences in the standardisation of 3D CoSeedis™ applications. The customized systems will implement your cell line of choice into the 3D CoSeedis™ platform. Please contact us to discuss your requirements.
Key Features of 3D CoSeedis™ Matrix
Modular Set-up
- Contact or distance co-cultures with feeder cells
- Combining up to 3 cell types to analyse interactions
- Serum, serum-reduced or serum-free conditions possible
- Application of tissue specific ECM components
Agarose-based Permeable Matrix
- Allows long term 3D culture of cell aggregates
- Sufficient supply with nutrients, oxygen, and catabolites – efficient disposal of toxic waste products
- Efficient use of physically separated feeder cells (distance co-culture)
- Cultivation in standard or defined media
Multi-purpose Applicability
- Applicable to various cell types
- Supports the formation of spheroidal and non-spheroidal aggregates
- Suitable for high-throughput and high-content applications
Process Directed Workflow Protocols
- Validated protocols
- Standardised and optimised components
- Highly reproducible 3D cell constructs
- Suitable for high-throughput and high-content applications
| Feature | Competition | 3D CoSeedis™ |
|---|---|---|
| Distance co-cultures | - | + |
| Aggregation of non-spheroidal cells | - | + |
| Long term culture | - | + |
| Easy handling | - | + |
| Easy harvesting of spheroids | + | + |
| HTS & HCS compatibility | - / + | + |
| Fully validated workflows/systems | - | + |
Applicable Cell Systems
These cells have been tested successfully for aggregate spheroid formation by our academic partners.
Details are described in the publication: A deep conical agarose microwell array for adhesion independent three-dimensional cell culture and dynamic volume measurement Andreas R. Thomsen et al., Lab Chip, DOI 10.1039/C7LC00832E.
Standardized Protocols for 3D Organoid Systems
The modular composition of the 3D CoSeedis ™ system allows distance co-cultures and, consequently, the standardisation of protocols. abc biopply’s product portfolio contains already standardised protocols for a wide variety of co-cultures, especially for the cultivation of different tumours.
Various application notes have been developed that constantly extend the range of assays in which 3D CoSeedis™ has been successfully implemented. Check out the latest notes:
High-Throughput / High-Content Screening Applications
3D CoSeedis™ is an ideally suited production tool to grow spheroidal 3D cell constructs in large scale, particularly for the use in HTS/HCS applications. With the various formats, 3D CoSeedis™ Chip200, 880 and 680, it is possible to grow up to 1’200, 5’280, and 16’320 spheroids per plate, respectively.
The major advantages for subsequent HTS/HCS are:
- Highly reproducible and homogeneous 3D cell aggregates across chips and plates.
- Substantial reduction of cell culture work > 5’000 3D constructs per 6-well plate; >16’000 per 24-well plate, arbitrarily upscalable.
- Increased screening efficiency through highly homogenous 384-well plates seeded with 1 to several 3D constructs / well.
- Standardized to work under defined conditions (e.g. serum-free aggregation and growth media).
- Long-term studies possible: up to 70 days in culture.
- Dual and sequential treatments possible over extended period of time with lots of up to 900 cell aggregates.
- 3D CoSeedis™ chips consist of agarose => biologically inert carrier matrix for optimal maintenance of primary and stem cells.
- Fully validated for a wide range of tumour 3D aggregates.
- Modular set-up (e.g. distance co-cultures) significantly extends the range of testable cells in 3D including primary cells and iPS.
- Fully validated read-outs:
- Growth monitoring
- Viability
- Cytotoxicity
- Histology: paraffin and frozen
- Colony forming assay
Research Applications
3D CoSeedis™ has been validated for various applications and according protocols have been developed. The different application notes can be downloaded here or contact us for further information.
Colony Forming Assay
A new approach to the colony forming assay in cancer research. 3D CoSeedis™ simplifies and facilitates the set-up, read-out and analysis of the assay substantially.
For further details you may also refer to the following Whitepaper.
Viability / Cytotoxicity
The application note addresses viability and/or cytotoxicity of 3D cell constructs grown in 3D CoSeedis™. The protocol describes an extension to all existing 3D CoSeedis™ systems and can be implemented easily without further requirements in equipment or appliances.
Cryo-embedding
3D CoSeedis™ offers an easy and elegant way to embed 3D constructs for cryo-sectioning without the need to manipulate individual spheroids. It reduces tricky and cumbersome manipulations and prevents damage or loss of 3D constructs.
Request more information about the CoSeedis System
The supporting documents available for this product can be downloaded below.