Do you need increased sensitivity and specificity from your RNA-seq experiments? Do you have ever-decreasing amounts of input RNA?
INTRODUCING NEBNEXT ULTRA II FOR RNA
Do you need increased sensitivity and specificity from your RNA-seq experiments? Do you have ever-decreasing amounts of input RNA? Our next generation of RNA library prep kits have been reformulated at each step, resulting in several fold higher yields of high quality libraries, enabling use of lower input amounts and fewer PCR cycles.
The new NEBNext Ultra II RNA kits from New England Biolabs (NEB) have streamlined, automatable workflows and are available for directional (strand-specific, using the "dUTP method") and non-directional library prep and are compatible with poly(A) mRNA enrichment or rRNA depletion. The kits are available with the option of SPRISelect beads for size-selection and clean-up steps.
Get more of what you need, with the highest library yields
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Generate high-quality libraries even with limited amounts of RNA:
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10 ng – 1 µg Total RNA (poly(A) mRNA workflow)
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5 ng – 1 µg Total RNA (rRNA depletion workflow)
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Increase the complexity and transcript coverage of your libraries
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Maximize flexibility by ordering reagents for your specific workflow needs, including rRNA depletion and poly(A) mRNA isolation reagents, and adaptors and primers (12-, 96-, and dual index) sold separately
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Rely on robust performance, even with low quality RNA, including FFPE
NEBNext Ultra II Directional RNA produces the highest yields, from a range of input amounts
Poly(A)-containing mRNA was isolated from 10 ng, 100 ng and 1 µg of Universal Human Reference RNA (Agilent® #740000) and libraries were made using the NEBNext Ultra II Directional RNA Kit (plus the NEBNext poly(A) mRNA Magnetic Isolation Kit), Kapa Stranded mRNA-Seq Kit, Kapa mRNA HyperPrep Kit and Illumina TruSeq Stranded mRNA Kit. The input RNA amount and number of PCR cycles are indicated. Library yields from an average of three replicates are shown. View more yield data.
NEBNext Ultra II Directional RNA libraries provide uniform GC content distribution, at a broad range of input amounts
Poly(A)-containing mRNA was isolated from Universal Human Reference RNA (Agilent #740000), and libraries were made using the NEBNext Ultra II Directional RNA Kit (plus the NEBNext Poly(A) mRNA Magnetic Isolation Module), Illumina TruSeq Stranded mRNA Kit, Kapa Stranded mRNA-Seq Kit and Kapa mRNA HyperPrep Kit. Libraries were sequenced on an Illumina NextSeq® 500 using paired-end mode (2x76 bp). Reads were mapped to the hg19 reference genome. GC content distribution for each library was calculated using mapped reads. Ultra II Directional RNA libraries had uniform GC content distribution across a range of input amounts, whereas for other kits the GC content distribution changed with different input amounts, indicating the introduction of input-dependent sequence bias. View more library data.
NEBNext Ultra II Directional RNA produces the highest yields with FFPE RNA
Ribosomal RNA was depleted from human adult normal liver tissue FFPE Total
RNA (Biochain # R2234149. RIN 2.5) and libraries were made using NEBNext Ultra II Directional RNA Kit (plus the NEBNext rRNA Depletion Kit (Human/Mouse/Rat)), Kapa Stranded RNA-Seq Kit with RiboErase, Kapa HyperPrep Kit with RiboErase, and Illumina TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero™ Gold. The input RNA amount and number of PCR cycles are indicated. Library yields from an average of three replicates are shown. View more FFPE data.