Product Pathways - DNA Damage
ATR Antibody #2790
|2790S||100 µl (10 western blots)||---||In Stock||---|
|2790||carrier free and custom formulation / quantity||email request|
|W||1:1000||Human, Mouse, Rat, Hamster, Monkey||Endogenous||300||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Species predicted to react based on 100% sequence homology: Bovine, Dog.
Specificity / Sensitivity
ATR Antibody detects endogenous levels of total ATR protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to central residues of human ATR. Antibodies are purified by protein A and peptide affinity chromatography.
Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are PI3 kinase-related kinase (PIKK) family members that phosphorylate multiple substrates on serine or threonine residues that are followed by a glutamine in response to DNA damage or replication blocks (1-3). Despite the essential role of ATR in cell cycle signaling and DNA repair processes, little is known about its activation. While there have been no published reports of phosphorylation sites on ATR, Cell Signaling Technology has produced an antibody directed against phospho-ATR (Ser428) that demonstrates in vivo and UV-induced phosphorylation of this protein. This reagent could prove to be a valuable tool for monitoring ATR activation. Proline-directed phosphorylation sites like this one are often targeted by CDKs and MAPKs and can often dramatically affect protein conformation (4,5). Threonine 1989 was recently identified as a potential marker of activated ATR (6,7).
- Kastan, M.B. and Lim, D.S. (2000) Nat Rev Mol Cell Biol 1, 179-86.
- Abraham, R.T. (2004) DNA Repair (Amst) 3, 883-7.
- Shechter, D. et al. (2004) DNA Repair (Amst) 3, 901-8.
- Pinna, L.A. and Ruzzene, M. (1996) Biochim Biophys Acta 1314, 191-225.
- Zhou, X.Z. et al. (1999) Cell Mol Life Sci 56, 788-806.
- Nam, E.A. et al. (2011) J Biol Chem 286, 28707-14.
- Liu, S. et al. (2011) Mol Cell 43, 192-202.
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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