Product Pathways - Metabolism
C/EBPα (D56F10) XP® Rabbit mAb #8178
|8178S||100 µl (10 western blots)||---||In Stock||---|
|8178P||40 µl (4 western blots)||---||In Stock||---|
|8178||carrier free and custom formulation / quantity||email request|
|W||1:1000||Human, Mouse||Endogenous||42, 28||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry)
Species predicted to react based on 100% sequence homology: Rat.
Specificity / Sensitivity
C/EBPα (D56F10) XP® Rabbit mAb recognizes endogenous levels of total C/EBPα protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala176 of human C/EBPα protein.
Western blot analysis of extracts from Hep G2 and LNCaP cells using C/EBPα (D56F10) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse lung using C/EBPα (D56F10) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded cell pellets, THP-1 (left) or Jurkat (right), using C/EBPα (D56F10) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using C/EBPα (D56F10) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Immunohistochemical analysis of paraffin-embedded human tonsil using C/EBPα (D56F10) XP® Rabbit mAb.
Confocal immunofluorescent analysis of THP-1 (left) and Jurkat (right) cells using C/EBPα (D56F10) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
CCAAT/enhancer-binding proteins (C/EBPs) are a family of transcription factors that are critical for cellular differentiation, terminal function, and inflammatory response (1). Six members of the family have been characterized (C/EBPα, β, δ, γ, ε, and ζ) and are distributed in a variety of tissues (1). Translation from alternative start codons results in two isoforms of C/EBPα (p42 and p30), which are both strong transcriptional activators (2). It has been reported that insulin and insulin-like growth factor-I stimulate the dephosphorylation of C/EBPα, which may play a key role in insulin-induced repression of GLUT4 transcription (3). Phosphorylation of C/EBPα at Thr222, Thr226, and Ser230 by GSK-3 seems to be required for adipogenesis (4).
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 9998 BSA
- 7727 Biotinylated Protein Ladder Detection Pack
- 2843 C/EBPalpha (p42) Antibody
- 3082 C/EBPbeta Antibody
- 2295 C/EBPα Antibody
- 3087 C/EBPβ (LAP) Antibody
- 2318 C/EBPδ Antibody
- 5554 CHOP (D46F1) Rabbit mAb
- 2895 CHOP (L63F7) Mouse mAb
- 9999 Nonfat Dry Milk
- 5425 Normal Goat Serum
- 3081 Phospho-C/EBPbeta (Ser105) Antibody (Rat Specific)
- 2841 Phospho-C/EBPα (Ser21) Antibody
- 2844 Phospho-C/EBPα (Thr222/226) Antibody
- 3084 Phospho-C/EBPβ (Thr235) Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 6883 SignalFire™ ECL Reagent
- 12757 SignalFire™ Elite ECL Reagent
- 12630 SignalFire™ Plus ECL Reagent
- 8112 SignalStain® Antibody Diluent
- 8114 SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
- 8059 SignalStain® DAB Substrate Kit
- 9997 Tris Buffered Saline with Tween® 20 (TBST-10X)
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
DRAQ5 is a registered trademark of Biostatus Limited.
SignalStain is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.