Product Pathways - Lymphocyte Signaling
BATF (D7C5) Rabbit mAb #8638
|8638S||100 µl (10 western blots)||---||In Stock||---|
|8638||carrier free and custom formulation / quantity||email request|
|W||1:1000||Human, Mouse||Endogenous||15||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Specificity / Sensitivity
BATF (D7C5) Rabbit mAb detects endogenous levels of total BATF protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human BATF protein.
Western blot analysis of extracts from various cell lines using BATF (D7C5) Rabbit mAb. Cell Line Source: Dr Abraham Karpas at the University of Cambridge.
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct encoding full-length human BATF (hBATF; +), using BATF (D7C5) Rabbit mAb.
Confocal immunofluorescent analysis of KARPAS-299 (left) and HeLa (right) cells using BATF (D7C5) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Cell Line Source: Dr Abraham Karpas at the University of Cambridge.
Basic leucine zipper transcriptional factor ATF-like (BATF) is a basic leucine zipper (bZIP) transcription factor and is part of the AP-1/ATF family that forms inhibitory dimers with members of the Jun family (1-3). Expression of BATF is largely restricted with highest levels found in mature T cells, and it is induced in B cells following immune responses including viral infection (1,2). BATF expression is also induced by IL-6 via a Stat3-dependent mechanism (4). BATF plays an important role in the differentiation of immune cell lineages (5-7). Studies of BATF-deficient mice have demonstrated a critical role for BATF in the formation of IL-17-expressing Th17 cells, in part, by regulating the expression of IL-17 (5,6). BATF knockouts are resistant to experimental autoimmune encephalomyelitis (EEA), consistent with the role of Th17 cells in this model for autoimmunity (5). Additional studies have found that BATF is important in generating antibody class switching. BATF is required for the generation of follicular helper T cells (Tfh), by regulating BCL6 and c-Maf (6,7). In B cells, BATF controls the expression of activation-induced cytidine deaminase (AID) and regulates class-switched antibody responses (7). Taken together, these studies suggest that BATF is a key regulator of distinct populations of immune cells.
- Dorsey, M.J. et al. (1995) Oncogene 11, 2255-65.
- Hasegawa, H. et al. (1996) Biochem Biophys Res Commun 222, 164-70.
- Echlin, D.R. et al. (2000) Oncogene 19, 1752-63.
- Senga, T. et al. (2002) Oncogene 21, 8186-91.
- Schraml, B.U. et al. (2009) Nature 460, 405-9.
- Betz, B.C. et al. (2010) J Exp Med 207, 933-42.
- Ise, W. et al. (2011) Nat Immunol 12, 536-43.
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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