Product Class: Kit

NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 1)

Note that additional index primers for multiplexing are provided with kit NEB #E7580 and NEB #E7560.

Product Introduction

The unique workflow of the NEBNext® Small RNA library prep kits addresses the challenge of minimization of adaptor-dimers while achieving production of high-yield, diverse multiplex libraries in a simple protocol.

  • Minimized adaptor-dimer contamination
  • High yields
  • Input RNA can be Total RNA
  • Suitable for methylated small RNAs (e.g., piRNAs) as well as unmethylated small RNAs
Catalog # Size Concentration
E7300S 24.0 reactions
E7300L 96.0 reactions

Protocols, Manuals & Usage

Protocols

  1. Protocol for use with NEBNext Small RNA Library Prep Set for Illumina (E7300, E7580, E7560, E7330)
  2. 6A. Quality Control Check and Size Selection using 6% Polyacrylamide Gel  NEBNext Small RNA Library Prep Set for Illumina (E7300, E7580, E7560, E7330)
  3. 6B. Quality Control Check and Size Selection using Pippin Prep NEBNext Small RNA Library Prep Set for Illumina (E7300, E7580, E7560, E7330)
  4. 6C. Quality Control Check and Size Selection using AMPure XP Beads NEBNext Small RNA Library Prep Set for Illumina (E7300, E7580, E7560, E7330)

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

Application Notes

FAQs & Troubleshooting

FAQs

  1. Is it possible to prevent abundant, unwanted RNAs to be included in the Small RNA library?
  2. Can I use Total RNA to make small RNA libraries or do I have to isolate or enrich the sample for small RNA?
  3. Why does the RT primer hybridization occur before 5’adaptor ligation?
  4. Do I have to hybridize the RT primer again after 5’ ligation?
  5. How are the barcodes introduced in the Multiplex libraries?
  6. During size selection on 6% PAGE gel, which bands should I cut out of the gel?
  7. Are libraries prepared with the NEBNext® Small RNA Library prep kit for Illumina compatible with paired-end flow cells for cluster generation?
  8. Can I use the small RNA sample preparation kit for Directional-RNA sequencing?
  9. What is the sequence of the final PCR product?
  10. Can I use enriched small RNA instead of total RNA for the NEBNext Small RNA Library Prep for Illumina?
  11. What should I do if my Small RNA may not have 5´ Phosphate and 3´ OH groups?
  12. How should my NEBNext Small RNA library be trimmed?
  13. Are the Monarch RNA Cleanup Kits compatible with NEBNext reagents for RNA library prep?
  14. What should I do if the sample sheet from the NEB.com website is not compatible with the software version I am using?
  15. Do you have Sample Sheets for use with the NEBNext Multiplex Small RNA Library Prep for Illumina?
  16. Are NEBNext adaptor and primers for Illumina compatible with NEBNext reagents for Illumina library preparation?

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