Product Pathways - Cytoskeletal Signaling
ROCK2 (D1B1) Rabbit mAb #9029
|9029S||100 µl (10 western blots)||---||In Stock||---|
|9029||carrier free and custom formulation / quantity||email request|
|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||160||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Species predicted to react based on 100% sequence homology: Hamster, Dog.
Specificity / Sensitivity
ROCK2 (D1B1) Rabbit mAb recognizes endogenous levels of total ROCK2 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ROCK2 protein.
Western blot analysis of extracts from various cell lines using ROCK2 (D1B1) Rabbit mAb.
ROCK (Rho-associated kinase), a family of serine/threonine kinases, is an important downstream target of Rho-GTPase and plays an important role in Rho-mediated signaling. Two isoforms of ROCK have been identified: ROCK1 and ROCK2. ROCK is composed of N-terminal catalytic, coiled-coil, and C-terminal PH (pleckstrin homology) domains. The C-terminus of ROCK negatively regulates its kinase activity (1,2). Caspase-3-induced cleavage of ROCK1 and direct cleavage of ROCK2 by granzyme B (grB) activates ROCK and leads to phosphorylation of myosin light chain and inhibition of myosin phosphatase (3). This phosphorylation may account for the mechanism by which Rho regulates cytokinesis, cell motility, cell membrane blebbing during apoptosis, and smooth muscle contraction (4-6).
- Nakagawa, O. et al. (1996) FEBS Lett. 392, 189-193.
- Lee, J.H. et al. (2004) J. Cell. Biol. 167, 327-337.
- Sebbagh, M. et al. (2005) J. Exp. Med. 201, 465-471.
- Amano, M. et al. (1996) J. Biol. Chem. 271, 20246-20249.
- Kureishi, Y. et al. (1997) J. Biol. Chem. 272, 12257-12260.
- Totsukawa, G. et al. (2000) J. Cell Biol. 150, 797-806.
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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