DescriptionNew England Biolabs supplies a 5X reaction buffer for use with Quick T4 DNA Ligase. At a 1X concentration this reaction buffer assures optimal activity of the enzyme.
Properties and Usage
6% Polyethylene glycol (PEG 6000)
Quality Control AssaysThe following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page.
- Endonuclease Activity (Nicking):
The product is tested in a reaction containing a supercoiled DNA substrate. After incubation for 4 hours the percent converted to the nicked form is determined by agarose gel electrophoresis.
- Non-Specific DNase Activity (16 hour):
The product is tested for non-specific nuclease degradation in a reaction containing a DNA substrate. After incubation for 16 hours there is no detectable degradation of the DNA substrate as determined by agarose gel electrophoresis.
- Phosphatase Activity (PNPP):
The product is tested in a reaction containing a p-nitrophenyl phosphate (PNPP), a chromogenic substrate for most phosphatases. After incubation there is no detectable phosphatase activity as determined by spectrophotometric analysis of released p-nitrophenol at 405 nm.
- RNase Activity (16 Hour Digestion):
The product is tested in a reaction containing a RNA substrate. After incubation for 16 hours there is no detectable degradation of the RNA substrate as determined by gel electrophoresis.