Product Class: Other

5´ Deadenylase
NEB1 recombinant 30 70 Heat

Product Introduction

5´ Deadenylase is capable of deadenylation from 5´-end of DNA and RNA, leaving the phosphate at 5´-end. It also cleaves AppppA into ATP and AMP. 

  • Isolated from a recombinant source
  • Supplied with 10X reaction buffer
  • Tested for the absence of DNases, RNases, and phosphatases
Catalog # Size Concentration
M0331S 2500.0 units 50000 units/ml

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Product Information

Description

Yeast 5´ Deadenylase is a member of the HIT (histidine triad) family proteins and specifically a member of the Hint branch. It is the yeast orthologue of aprataxin. Mutations in human aprataxin have been known to be involved in the neurological disorder known as ataxia oculomotor apraxia-1 (1). The human protein has been shown to resolve abortive ligation intermediates by removing the AMP at the 5´ end of DNA (AMP-DNA hydrolase activity) (2). It also repairs DNA damage at 3´ ends by removing 3´-phosphate and 3´-phosphoglycolate (3). Human aprataxin acts on small molecules as well, nucleotide polyphosphates such as diadenosine tetraphosphate (AppppA) as well as lysyl-AMP. 

The 5´ Deadenylase is encoded by HNT3 gene of S. cerevisiae. NEB has shown this protein is capable of deadenylation from 5´ end of DNA and RNA, leaving the phosphate at 5´ end. It also cleaves AppppA into ATP and AMP. Its activity on lysyl-AMP is not detectable.

Product Source

Purified from an E. coli strain carrying a plasmid encoding 5´ Deadenylase from S. cerevisiae.
This product is related to the following categories:
RNA Modification

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Unit Definition

One unit is defined as the amount enzyme required to remove 10 pmoles of AMP from a 5'-adenylated DNA oligo in 10 minutes at 30°C.

Reaction Conditions

1X NEBuffer™ 1
Incubate at 30°C

1X NEBuffer™ 1
10 mM Bis-Tris-Propane-HCl
10 mM MgCl2
1 mM DTT
(pH 7 @ 25°C)

Storage Buffer

10 mM Tris-HCl
100 mM NaCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
0.1% (w/v) Triton® X-100
pH 7.5 @ 25°C

Heat Inactivation

70°C for 20 minutes

Product Notes

  1. 5'-adenylated DNA could be dsDNA, ssDNA and nicked DNA with the adenylate residue at the 5' terminus of the nick. Deadenylation is more efficient with dsDNA and nicked DNA.

References

  1. Le Ber, I. et al. (2003). Brain. 126, 2761.
  2. Ahel, I. et al. (2006). Nature. 443, 713.
  3. Takahashi, T. et al. (2007). Nucl. Acids Res. 35, 3797.

Protocols, Manuals & Usage

Protocols

  1. A Typical Deadenylation Reaction (M0331)

FAQs & Troubleshooting

FAQs

  1. What is the molecular weight of 5’ Deadenylase?
  2. Is 5’ Deadenylase a tagged protein?
  3. Can 5’ Deadenylase deadenylate ssDNA?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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