Product Class: Other

Random Primer 6

Product Introduction

  • Random Primer 6 is a mixture of random hexanucleotides is used to prime DNA synthesis in vitro along multiple sites of denatured template DNA
  • Ensures the presence of virtually all sequence combinations of hexamer primers
  • Used to screen gene libraries
  • Used to probe northern and southern blots
  • Used for in situ hybridizations
  • Sequence: 5' d(N6 ) 3' [N=A,C,G,T]
Catalog # Size Concentration
S1230S 1.0 A260 units

Product Information

Description

This mixture of random hexanucleotides is used to prime DNA synthesis in vitro along multiple sites of denatured template DNA (1). This primer-template complex is a suitable substrate for DNA Polymerase I (Klenow Fragment). The newly synthesized complementary DNA is "oligo-labelled" by substituting any radiolabelled nucleotide for the appropriate nonradioactive nucleotide in the reaction mixture. Use of synthetic d(N)6 primer ensures the presence of virtually all sequence combination of hexamer primers which results in equally labelled DNA of high specific activity (1,2). Oligolabelling by this method generates probes which can be used to screen gene libraries (3), probe Southern and Northern blots (4,5), and for in situ hybridizations (6).

Sequence

5' d(N6 ) 3' [N=A,C,G,T]
This product can be used in the following applications:
RT-PCR & cDNA Synthesis

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Product Notes

  1. For use in standard Random Priming Reaction, dissolve 33 µg of (NEB #S1230S) in 80 µl of water or 10X labeling buffer.
  2. 18.6 nmol of S1230 are supplied.

References

  1. Feinberg, A.P. and Vogelstein, B. Anal. Biochem.. 132,
  2. Feinberg, A.P. and Vogelstein, B. Anal. Biochem.. 137,
  3. Grunstein, M. and Hogness, D.S. Proc. Natl. Acad. Sci. USA. 72,
  4. Southern, E.M. J. Mol. Biol.. 98,
  5. Smith, G.E. and Summers, M.D. Anal. Biochem.. 109,
  6. Hasse, A. et al. Methods Virol.. 7,

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.