Product Class: Restriction Endonuclease

FokI
neb31 cloned at NEB recombinant 2site dil_A 37 65 Heat CpG dcm

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

NEB restriction endonuclease that recognizes the sequence GGATGNNNNNNNNN^NNNN_

Product Introduction

  • 100% activity in rCutSmart Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests
  • Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence
  • Requires two or more sites for cleavage. Please review the enzyme list and recommendations in the following table as well as the background information article.
  • Restriction Enzyme Cut Site: GGATG(9/13)
Catalog # Size Concentration
R0109S 1000.0 units 5000 units/ml
R0109L 5000.0 units 5000 units/ml

Protocols, Manuals & Usage

Protocols

  1. Optimizing Restriction Endonuclease Reactions
  2. Restriction Digest Protocol
  3. Double Digest Protocol with Standard Restriction Enzymes

Usage & Guidelines

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. Does FokI tend to degrade DNA?
  2. Why does FokI degrade DNA?
  3. Is extended digestion of FokI recommended?
  4. Is FokI blocked by methylation?
  5. Is FokI used in special techniques?
  6. What is the molecular weight of FokI?
  7. Is FokI activity sensitive to pH?
  8. Is FokI active at 25°C?
  9. Does FokI cleave ssDNA?
  10. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?

Troubleshooting