Product Class: Vector


Catalog #SizeConcentration
N3022S30 μg1,000 μg/ml
N3022L150 μg1,000 μg/ml


This is the double-stranded, nicked, circular form of ΦX174 DNA (relaxed form). The molecular weight of ΦX174 DNA is 3.50 X 106 daltons it is 5,386 base pairs in length. Greater than 90% of the molecules are RF II, the remainder are RF I (supercoiled) and linear ΦX174 DNA.

DNASU and Addgene are central repositories for plasmid clones and collections that may also be helpful.

Product Source

ΦX174 RF II is prepared by relaxing (nicking) ΦX174 RF I DNA in high salt at 75°C. Greater than 90% of the molecules are in the RF II form as indicated by agarose gel electrophoresis.

Properties and Usage

Affinity Tag


Storage Temperature


Storage Conditions

10 mM Tris-HCl
pH 8.0 @ 25°C

Supporting Documents

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at or fill out the Technical Support Form for appropriate document.


  1. Sanger, F. et al. (1978). J. Mol. Biol.. 125, 225-246.
  2. Freifelder, D. and Dewitt, R. (1977). Gene. 1, 385.