DescriptionThis is the double-stranded, nicked, circular form of ΦX174 DNA (relaxed form). The molecular weight of ΦX174 DNA is 3.50 X 106 daltons it is 5,386 base pairs in length. Greater than 90% of the molecules are RF II, the remainder are RF I (supercoiled) and linear ΦX174 DNA.
DNASU is a central repository for plasmid clones and collections that may also be helpful.
Product SourceΦX174 RF II is prepared by relaxing (nicking) ΦX174 RF I DNA in high salt at 75°C. Greater than 90% of the molecules are in the RF II form as indicated by agarose gel electrophoresis.
Properties and Usage
10 mM Tris-HCl
1 mM EDTA
pH 8.0 @ 25°C
- Sanger, F. et al. (1978). J. Mol. Biol.. 125, 225-246.
- Freifelder, D. and Dewitt, R. (1977). Gene. 1, 385.