This is the double-stranded, nicked, circular form of ΦX174 DNA (relaxed form). The molecular weight of ΦX174 DNA is 3.50 X 106 daltons it is 5,386 base pairs in length. Greater than 90% of the molecules are RF II, the remainder are RF I (supercoiled) and linear ΦX174 DNA.
DNASU and Addgene are central repositories for plasmid clones and collections that may also be helpful.
ΦX174 RF II is prepared by relaxing (nicking) ΦX174 RF I
DNA in high salt at 75°C. Greater than 90% of the molecules are in the RF II
form as indicated by agarose gel electrophoresis.
Properties and Usage
10 mM Tris-HCl 1 mM EDTA pH 8.0 @ 25°C
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