DescriptionThis is the double-stranded, covalently closed, circular form of ΦX174 RF I DNA (supercoiled). The molecular weight of ΦX174 RF I is 3.50 x 106 daltons and it is 5,386 base pairs in length. Greater than 90% of the molecules are RF I, and the remainder are RF II, as determined by agarose gel electrophoresis.
DNASU is a central repository for plasmid clones and collections that may also be helpful.
Peptide SequenceThe ΦX174 RF I DNA is isolated from E. coli HF 4704 infected with ΦX174 am3 cs70 by a modification of the method of Godson and Vapnek (nucleotide sequenced by F. Sanger).
Properties and Usage
10 mM Tris-HCl
1 mM EDTA
pH 8.0 @ 25°C
Material Safety Datasheets
- Sanger, F. et al. (1978). J. Mol. Biol.. 125, 225-246.
- Godson, G.N. and Vapnek, D. (1973). Biochem. Biophys. Acta. 299, 516.