This is the double-stranded, covalently closed, circular form of ΦX174 RF I DNA (supercoiled). The molecular weight of ΦX174 RF I is 3.50 x 106 daltons and it is 5,386 base pairs in length. The vast majority of the molecules are RF I,
and the remainder are RF II, as determined by
agarose gel electrophoresis.
DNASU is a central repository for plasmid clones and collections that may also be helpful.
The ΦX174 RF I DNA is isolated from E. coli HF 4704
infected with ΦX174 am3 cs70 by a modification of the method of
Godson and Vapnek (nucleotide sequenced by F. Sanger).
Properties and Usage
10 mM Tris-HCl 1 mM EDTA pH 8.0 @ 25°C
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at firstname.lastname@example.org or fill out the Technical Support Form for appropriate document.