Product Class: Kit

Quick Ligation™ Kit

Product Introduction

Quick Ligase will ligate these substrates:

dsDNA




Nicked DNA/RNA





 

The Quick Ligation™ Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature. (25°C )

Catalog # Size Concentration
M2200S 30.0 reactions
M2200L 150.0 reactions

Protocols, Manuals & Usage

Protocols

  1. Protocol for the Quick Blunting Kit (E1201)
  2. Transformation Protocol
  3. Quick Ligation Protocol (M2200)

Usage & Guidelines

Application Notes

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. What factors can cause incomplete ligation and/or transformation using the Quick Ligation Kit?
  2. Why is my transformation not working and what control reactions should be run?
  3. What is the concentration of T4 DNA Ligase provided in the Quick Ligation Kit?
  4. Can conventional T4 DNA Ligase (400,000 units/ml) be used with the Quick Ligation buffer?
  5. How to calculate the molarity of ends?
  6. The protocol for Quick Ligation Kit indicates the reaction should not beheat inactivated. How can I inactivate the ligation activity?

Troubleshooting